Unexpected Conversion of Epinephrine into Tetrahydroisoquinolines in a Solution containing Ascorbic Acid

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概要

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• Degradation of ^3H-epinephrine during incubation for 14 h at 37℃ in a phosphate buffer, pH 7.0,containing ascorbic acid was observed in a study on the radioimmunoassay of epinephrine. Chromatographic separations of the products were carried out on a large scale. They were identified as 1,2,3,4-tetrahydro-4,6,7-trihydroxy-2-methylisoquinoline (1) and 1,2,3,4-tetrahydro-4,7,8-trihydroxy-2-methylisoquinoline (2). These products were synthesized from epinephrine and formaldehyde for the first time. Our results suggested that ^3H-epinephrine reacted with formaldehyde generated through the oxidation of ascorbic acid by oxygen from air under the incubation conditions used above to yield 1 or 2. Care is clearly required in using ascorbic acid as an antioxidant in catecholamine studies.

• 公益社団法人日本薬学会の論文
• 1982-04-25

著者

• 田村 善蔵

  Faculty of Pharmaceutical Sciences, University of Tokyo

• 田村 善蔵

  東大薬

• 白幡 晶

  Faculty Of Pharmaceutical Sciences University Of Tokyo:(present Address)tokyo Biochemical Research I

• 吉岡 正則

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• 田村 善蔵

  Faculty Of Pharmaceutical Sciences University Of Tokyo

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