Simultaneous Determination of Multiple Enzyme Activities by High Performance Liquid Chromatography
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概要
- 論文の詳細を見る
A method is proposed for the simultaneous determination of multiple enzyme activities in a specimen by high performance liquid chromatography. To examine the applicability of the method, the hydrolytic activities of arylsulfatase, β-glucosidase and β-glucuronidase, all having broad optimal pH ranges around 5 were assayed by quantitative determination of 8-hydroxyquinoline (oxine, Ox), 5,7-dichloro-8-hydroxyquinoline (Di-Cl) and 5-chloro-7-iodo-8-hydroxyquinoline (chinoform, CF), the products released enzymatically from Ox-β-D-glucoside, Di-Cl-sulfate and CF-β-D-glucuronide. The method was applied to rat organs and Bifidobacterium infantis No. 9.
- 社団法人日本薬学会の論文
- 1979-08-25
著者
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中村 洋
Faculty Of Pharmaceutical Sciences University Of Tokyo
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田村 善蔵
Faculty of Pharmaceutical Sciences, University of Tokyo
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三浦 研二
Faculty of Pharmaceutical Sciences, University of Tokyo
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田中 博
Faculty of Pharmaceutical Sciences, University of Tokyo
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田村 善蔵
東大 薬
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田村 善蔵
東京大学薬学部薬品分析化学教室:(現)慶応義塾大学病院薬剤部
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田村 善蔵
Faculty Of Pharmaceutical Sciences University Of Tokyo
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三浦 研二
Faculty Of Pharmaceutical Sciences University Of Tokyo
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