Spectrophotometric Determination of Bunte Salts as Bisulfite with Acidbleached Basic Fuchsine or N-(p-Dimethylaminophenyl)-1,4-naphthoquinoneimine after Reduction with Dithiothreitol

概要

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Colorimetric and fluorometric methods for the determination of Bunte salts have been developed. Bunte salts are reduced with dithiothreitol (DTT) to monothiols and bisulfite. The latter is determined colorimetrically with a basic fuchsine reagent in the presence of mercuric chloride or fluorometrically with N-(p-dimethylaminophenyl)-1,4-naphthoquinoneimine in the presence of a mixture of p-chloromercuribenzoic acid and sodium arsenite. The sulfhydryl reagents and arsenite mask the thiols by forming mercaptides and a DTT-arsenite complex, respectively. The colorimetric and the fluorometric methods can determine Bunte salts in a range of 5×10^<-9> to 1×10^<-7> mol and 2×10^<-8> to 1.5×10^<-7> mol, respectively.
社団法人日本薬学会の論文
1977-09-25