The Production of Bisulfite from Pantetheine-S-sulfonic Acid by Bifidobacterium bifidum

概要

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Identification of the radioactive metabolite of PaS^<35>SO_3H in B. bifidum N4 was undertaken. The strain was incubated with (PaS^<35>SO_3)_2 Ca for 60 min at 37° in CySS (-) MGM, and after removal of the bacteria, the medium which showed a new radioactive peak on a chromatogram was neutralized and reacted with NEM or HCHO and PABA. By this treatment, the peak disappeared and in turn a new peak emerged in both cases. They were identified as NEM-^<35>SO_3H and SMPABA-^<35>S by PEP (1 M HCOOH, pH 2) and radiochemical crystallizations. In the absence of the bacteria, the peak of the radioactive metabolite was not produced by the incubation of (PaS^<35>SO_3)_2 Ca with CySS (-) MGM, neither produced NEM-^<35>SO_3H from (PaS^<35>SO_3)_2 Ca by NEM treatment. Furthermore, transformation of ^<35>SO_4^<2-> to H^<35>SO_3^- by the strain was not observed. These results demonstrate the production of HSO_3^- from PaSSO_3H by B. bifidum N4.
社団法人日本薬学会の論文
1974-07-25