Fluorescence Reaction of Bisulfite with Indophenol Blue and Its Application to Determination of Bisulfite
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概要
- 論文の詳細を見る
Bisulfite reacted with N-(p-dimethylaminophenyl)-1,4-naphthoquinoneimine (indophenol blue, I) to yield blue fluorescence in alkaline media. The reaction was dependent upon pH and temperature, being optimal at pH 3.5-4.0 and around 37°, and completed within 30 min. Based on these observations, a fluorometric method has been developed for the determination of bisulfite. A linear relationship was obtained in the range of 1.5×10^<-8>-1.5×10^<-7> mole of bisulfite. By column chromatography, thin-layer chromatography and paper electrophoresis, it was proved that at least two fluorescent compounds were formed in the reaction mixture and they were supposed to be bisulfite adducts of I containing one and two -SO_3H groups.
- 社団法人日本薬学会の論文
- 1975-06-25
著者
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中村 洋
Faculty Of Pharmaceutical Sciences University Of Tokyo
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田村 善蔵
Faculty of Pharmaceutical Sciences, University of Tokyo
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田村 善蔵
東大 薬
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田村 善蔵
東京大学薬学部薬品分析化学教室:(現)慶応義塾大学病院薬剤部
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田村 善蔵
Faculty Of Pharmaceutical Sciences University Of Tokyo
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