Assay of Arginine-esterase Activities by High Performance Liquid Chromatography
スポンサーリンク
概要
- 論文の詳細を見る
Arginine-esterase activities of trypsin, plasmin and kallikreins were assayed by high performance liquid chromatography (HPLC) with BAEE (or TAME) and DAME. 1. In the case of conventional method with BAEE (or TAME), only the hydrolyzed product of BA (or TA) was eluted from the column of "Zipax" SCX (20 cm×2.0 mm i.d.) with 0.1M phosphate buffer (pH 7.0), while BAEE (or TAME) was retained on the column. 10^<-3>-10^<-2> TAME units of these enzymes were easily measurable by determination of eluted product at several minutes intervals. 2. Using DAME as a fluorescent substrate, separation of DA from DAME was performed on the column of Hitachi gel #3010 (15 cm×2.0 mm i.d.) by elution of 20% (v/v) Tris-HCl buffer (0.05M, pH 8.5) in methanol. In this condition, 4 pmole of DA was determined and 10^<-5>-10^<-4> TAME units of these enzymes were measurable.
- 公益社団法人日本薬学会の論文
- 1975-05-25
著者
-
田村 善蔵
Faculty of Pharmaceutical Sciences, University of Tokyo
-
今成 登志男
Faculty Of Pharmaceutical Sciences Chiba University
-
田村 善蔵
東京大学医学部
-
田村 善蔵
Faculty Of Pharmaceutical Sciences University Of Tokyo
-
松本 宏治郎
Faculty of Pharmaceutical Sciences, University of Tokyo
-
松本 宏治郎
Faculty Of Pharmaceutical Sciences University Of Tokyo
関連論文
- 2-CARBOXY-1-HYDROXY-4-NAPHTHYLMETHYLDIMETHYLSULFONIUM CHLORIDE AS A NEW FLUORESCENT KOSHLAND REAGENT
- Fluorometric Determination of Biogenic Indole Compounds using Perchloric Acid
- Determination of Fluorescein and Fluorescein Monoglucuronide excreted in Urine
- Studies on the Metabolites of Fluorescein in Rabbit and Human Urine
- Simultaneous Determination of Multiple Enzyme Activities by High Performance Liquid Chromatography
- Supplemental Studies on Relationship between Structure and Spectrum of Fluorescein
- Spectrophotometric Determination of Bunte Salts as Bisulfite with Acidbleached Basic Fuchsine or N-(p-Dimethylaminophenyl)-1,4-naphthoquinoneimine after Reduction with Dithiothreitol
- Fluorescence Reaction of Bisulfite with Indophenol Blue and Its Application to Determination of Bisulfite
- A Simple Synthesis of Amino-containing Bunte Salts by the Reaction of Aminothiols with Chlorosulfonic Acid
- Spectrophotometric Determination of Bunte Salts
- Fluorometric Assay of Bisulfite
- The Production of Bisulfite from Pantetheine-S-sulfonic Acid by Bifidobacterium bifidum
- Studies on the Metabolites in Urine and Feces of Rat after Oral Administration of Radioactive Pantethine
- Gas Chromatographic Analysis of Disaccharides. II. Analysis of Disaccharides in Human Body Fluids
- Mechanism of Utilization of Pantetheine-S-Sulfonic Acid by Bifidobacterium bifidum
- Gas Chromatography of Catecholamines and Related Compounds
- Relationship between Neurological Symptoms and Concentrations of Clioquinol in Serum and Nervous Systems of Beagle Dogs
- Gas Chromatography and Its Combination with Mass Spectrometry of Urinary Sugar Alcohols
- Fluorometric Analysis of Pyruvic Acid with 4'-Hydrazino-2-stilbazole
- A New Ultra-micro Microbioassay
- Behaviour of Plasma Sugars after Oral Administration of Lactose
- Gas Chromatographic Analysis of Disaccharides. I. Analysis of Disaccharides as Trimethylsilyl and Trifluoroacethyl Derivatives of Corresponding Alditols
- Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. II. Specificity of the Method in Pyridoxal-5-phosphate Determination.
- Fluorometric Determination of Pyridoxal-5-phosphate and Pyridoxal in Biological Materials. I. Procedure and Application.
- Fluorometric Determination of Catecholamines on a Three-Phase Thin-Layer Plate
- Fluorometric Determination of Cyanide by the Reaction with Pyridoxal
- Reaction of Pyridoxal with Cyanide
- High-Performance Liquid Chromatographic Determination of L-3,4-Dihydroxyphenylalanine (L-DOPA) and Its Metabolites in the Urine of Patients with Parkinson's Disease, Control Patients and Normal Subjects after Oral Administration of L-DOPA
- Presence of (±)-δ-(3,4-Dihydroxyphenyl)-γ-valerolactone in Human Urine
- Gas Chromatography of Copper (II) and Nickel (II) Chelates of Some β-Ketoimine Derivatives of 2,4-Pentanedione and Salicylaldehyde
- Metal Complexes of D-Glucosamine and its Derivatives. VII. Metal Complexes of D-Glucosaminic Acid
- Metal Complexes of D-Glucosamine and its Derivatives. VI. Determinations of Stability Constants of Copper Complexes of Methyl-β-D-glucosaminide and of 3,4,6-Tri-O-methyl-D-glucosamine by pH Titration Method
- Metal Complexes of D-Glucosamine and its Derivatives. V. Spectrophotometric Investigations on Copper (II), Nickel (II), and Cobalt (II) Complexes of D-Glucosamine and the Preparation of Glucosamine-copper Complex
- Metal Complexes of D-Glucosamine and its Derivatives. IV. Determinations of Stability and Equilibrium Constants of Metal Complexes of D-Glucosamine by pH Titration Method
- Metal Complexes of D-Glucosamine and its Derivatives. III. Determinations of Acid Dissociation Constants of D-Glucosamine and its Three O-Methyl Derivatives
- 1.有害性金属のペーパークロマトグラフ法による定量法(第10回日本薬学大会衛生化学・分析化学シンポジウム(其の2)有害性金属の微量定量法)
- Semiquinone Radicals Generated from Catecholamines by Ultraviolet Irradiation
- 3-O-β-D-Glucopyranosiduronyl-D-glucaro-1,4-lactone
- Gas Chromatography of D-Glucaric Acid and Its Lactones, and Application to Measuring Their Transformation
- Synthesis of 5-Chloro-7-iodo-8-quinolinol (Chinoform) Glucuronide
- Studies on the Components of Clerodendron trichotomum THUNB. III. A New Glycoside, Acacetin-7-glucurono-(1→2)-glucuronide from the Leaves
- Selective Methanolysis of Lactone Rings in Some Acyl Carbohydrates
- Determination of Neutral and Amino Sugars in Glycoproteins by Gas Chromatography
- Serum Levels of 5-Chloro-7-iodo-8-quinolinol and Its Toxicity in Various Animals
- Hydrolysis of 5-Chloro-7-iodo-8-quinolinol Conjugates catalyzed by Metal Ions
- A Gas Chromatographic Determination Method of 5-Chloro-7-iodo-8-quinolinol and Its Conjugates in Biological Fluids
- Analysis of 5-Chloro-7-iodo-8-quinolinol Conjugates by High Performance Liquid Chromatography
- Assay of Arginine-esterase Activities by High Performance Liquid Chromatography
- A New Fluorometric Analysis of Dulcin using Sodium Nitrite. IV. Studies on the Reaction Mechanism
- Synthesis of 5-Chloro-7-iodo-8-quinolinol Sulfate
- A New Fluorometric Analysis of Dulcin using Sodium Nitrite. III. Structural Determination of the Fluorescent Compound by the Single Crystal X-Ray Diffraction Method
- A New Fluorometric Analysis of Dulcin using Sodium Nitrite. II. Isolation and Structural Investigation of the Fluorescent Compound
- Bifidus Factors in Carrot. I. Purification and Some Properties
- Simultaneous Determination of Urinary Uronic Acids and Saccharic Acids by Gas Chromatography
- Isolation and Structural Analysis of a Fluorescent Compound produced by the Reaction of Dulcin with Sodium Nitrite
- Selectivity of Utilization of Galactosyl-Oligosaccharides by Bifidobacteria
- Gas Chromatography of Trifluoroacetyl Derivatives of Alditols and Trimethylsilyl Derivatives of Aldonolactones
- Determination of Vanilpyruvic Acid in Urine by High-Speed Liquid Chromatography
- An Improved Method for the Millon Reaction
- Mechanism of the Millon Reaction. II. The Crystal Structure of Pigment 1
- Mechanism of the Millon Reaction. I. Isolation of Coloring Substances
- Improved Synthesis of Sulfoconjugate Isomers of Norepinephrine and Epinephrine, and Separation of All Sulfoconjugates of Catecholamines by Thin-Layer and High-Performance Liquid Chromatography
- Studies on the Metabolism and Excretion of L-3,4-Dihydroxyphenylalanine (L-DOPA) in Human Beings by Gas Chromatography
- The Plasma Levels of Dopa and Catecholamines after Oral Administration of L-Dopa
- Determination of Vanilmandelic Acid and Homovanillic Acid in Urine by Gas Chromatography
- Gas Chromatographic Determination of 3,4-Dihydroxyphenylalanine (DOPA) in Urine
- Fluorescence Assay of α-Oxo Acids with 4'-Hydrazino-2-stibazole
- Oligosaccharides and Polysaccharides specifically Utilizable by Bifidobacteria
- Determination of 3-Methoxytyramine, Normetanephrine and Metanephrine in Human Plasma by Gas Chromatography
- Synthesis of Norepinephrine-O-sulfates
- Catecholamines in Rat Tissues and Serum determined by Gas Chromatographic Method
- The Presence of Conjugated Dopamine in Normal Human Plasma
- Detection of Catecholamines after N-Dansylation on the Surface of Alumina
- Selective and Sensitive Method for Determination of Vanillylmandelic Acid (VMA) in Urine
- Actual Microanalysis by Large Volume Injection to Gas Chromatograph equipped with Electron Capture Detector
- Synthesis of Epinephrine Monosulfates
- Selective Condensation of Plasma Kinins from Biological Fluids with Siliconised Silica Gel
- Formation of Thiohydantoin Derivative of Proline from C-Terminal of Peptides
- Unexpected Conversion of Epinephrine into Tetrahydroisoquinolines in a Solution containing Ascorbic Acid
- Studies on Radioimmunoassay of Metanephrine
- A Simple Determination of Catechol-O-Methyltransferase Activity
- Preparation of Specific Antibodies to Catecholamines and L-3,4-Dihydroxy-phenylalanine. III. Preparation of Antibody to Epinephrine for Radioimmunoassay
- Preparation of Specific Antibodies to Catecholamines and L-3,4-Dihydroxyphenylalanine. II. The Site of Attachment on Catechol Moiety in the Conjugates
- Preparation of Specific Antibodies to Catecholamines and L-3,4-Dihydroxyphenylalanine. I. Preparation of the Conjugates
- Preparation of a Specific Antibody to Each One of Catecholamines and L-DOPA
- Bifidus Factors in Carrot. III. The Structure of The Factor in Fraction V
- Bifidus Factors in Carrot. II. The Structure of the Factor in Fraction IV
- A Simpler Synthesis of D-Pantothenic Acid 4'-Phosphate
- Metal Complexes of Isonicotinoylhydrazine and Related Compounds. III. Consecutive Formation Constants for Various Metal Ions by the pH Titration Method.
- Metal Complexes of Isonicotinoylhydrazine and Related Compounds. II. Acid Dissociation Constants and Ultraviolet Absorption Spectra of Isonicotinoylhydrazine and Related Compounds.
- New Fluorescence Edman Reagent for Microanalysis of Peptides
- The Total Amino Acid Sequence of Substance A produced by Streptomyces carzinostaticus
- The Amino Acid Sequence around Cystine Residue in Substance A produced by Streptomyces Carzinostaticus
- Gas Chromatography of the Catecholamines as Their Trifluoroacetates in Urine and Tumor
- Gas Chromatography of Catecholamines as Their Trifluoroacetates
- Gas Chromatography of Catecholamines using Dimethyl Sulfoxide as an Effective Solvent for Trimethylsilylation.
- Ultra-micro Analysis of Serum Protein in a Few Nanoliter of Samples
- 3-Ketoglucuronic Acid. II. Constitution and Chemical Properties of 3-Ketoglucuronic Acid
- 3-Ketoglucuronic Acid. I. Synthesis of 3-Ketoglucuronic Acid
- Synthesis of 3-Keto-D-glucuronic Acid