Gas Chromatographic Determination of 3,4-Dihydroxyphenylalanine (DOPA) in Urine

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概要

• 論文の詳細を見る

• Urinary 3,4-dihydroxyphenylalanine (DOPA) was extracted with alumina after removal of the fatty substances by shaking with ether-benzene (5 : 2), converted to n-butyl ester with n-butanol-HCl (5N) at 100°for 20 min, trifluoroacetylated with trifluoroacetic anhydride in ethyl acetate and determined by gas chromatography using electron capture detector. The overall recoveries of the added DOPA (50,75,100,and 150 ng) from urine (2 ml) were 66.0% ±3.55% (n=8). The pressence of DOPA in normal human urine was confirmed by gas chromatographymass spectroscopy.

• 公益社団法人日本薬学会の論文
• 1972-11-25

著者

• 田村 善蔵

  Faculty of Pharmaceutical Sciences, University of Tokyo

• 今井 一洋

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• 吉植 庄平

  Kyoritsu Women's University

• 田村 善蔵

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• 吉植 庄平

  Faculty of Pharmaceutical Sciencs, University of Tokyo

• 有住 伸子

  Faculty of Pharmaceutical Sciences, University of Tokyo

• 王 美代

  Faculty of Pharmaceutical Sciences, University of Tokyo

• 田村 善蔵

  Pharmaceutical Institute School Of Medicine Keio University

• 王 美代

  Faculty Of Pharmaceutical Sciences University Of Tokyo Hongo Bunkyo-ku Tokyo

• 有住 伸子

  Faculty Of Pharmaceutical Sciences University Of Tokyo

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