Organic Phosphates. IX. An Effective Separation of Flavin Nucleotides and Adenine Nucleotide from Hydrolysates of Flavin Adenine Dinucleotide

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概要

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• For the purpose of isolation and quantitative estimation of the hydrolysis products of FAD, ion exchange column chromatography was used. The hydrolysates were fractionated stepwise from Dowex-1 (Cl^-) with two systems of combined solvents : (1) Water, 0.002N hydrochloric acid, and 0.01N hydrochloric acid+0.04N ammonium chloride, and (2) 0.1N ammonium chloride and 0.1N ammonium chloride+0.01N hydrochloric acid. The former solvent system could effectively separate FR, AMP, FMP with FAD, and cyFMP, and the latter AMP, FMP with cyFMP, and FAD. The combined use of these two solvent systems was shown to effect clear-cut separation of the flavin nucleotides and adenine nucleotide contained in the hydrolysate of FAD.

• 社団法人日本薬学会の論文
• 1960-05-25

著者

• 入江 昌親

  Faculty of Pharmaceutical Sciences, Hoshi University

• 入江 昌親

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• 浮田 忠之進

  Faculty of Pharmaceutical Sciences, University of Tokyo

• 浮田 忠之進

  Faculty Of Pharmaceutical Sciences The University Of Tokyo

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