Different Behavior towards Raw Starch of Two Glucoamylases from Aspergillus saitoi

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概要

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• Two glucoamylases [EC 3.2.1.3] of Asperigillus saitoi, Gluc M_1 (molecular weight (M.W.) 90000) and Gluc M_2 (M.W.70000), which have similar pH optima and specific activities towards soluble starch were studied as to their behavior towards raw starch. The pH optima for raw starch digestion were different, 3.5 for Gluc M_1 and 4.0 for Gluc M_2. These enzymes digested raw starch almost completely but at quite different rates, Gluc M_2 being 51 times less effective than Gluc M_1. Gluc M_1 tightly bound to raw starch with maximum binding occurring at pH 3.0. The binding constant K of Gluc M_1 to raw starch at pH 3.0 and 4℃ was 1.6×10^5M<-1>. In contrast, Gluc M_2 which lacks the C-terminal region of Gluc M_1,had as small a K value as 3.2×10^3M<-1>, with practically no binding to the raw starch. These results indicate that only Gluc M_1 has an additional raw starch-binding site, besides the active center, in the C-terminal region.

• 社団法人日本薬学会の論文
• 1990-10-25

著者

• 入江 昌親

  Department of Microbiology, Hoshi College of Pharmacy

• 高橋 朋子

  School Of Pharmaceutical Sciences Toho University

• 中尾 悦子

  Institute Of Chemistry:hoshi University

• 高橋 朋子

  Institute of Medical Chemistry, Hoshi University

• 入江 昌親

  Faculty of Pharmaceutical Sciences, Hoshi University

• 池上 佳男

  Faculty of Pharmaceutical Sciences, Hoshi University

• 入江 昌親

  Faculty Of Pharmaceutical Sciences University Of Tokyo

• 入江 昌親

  Department Of Microbiology Hoshi College Of Pharmacy

• 池上 佳男

  Faculty Of Pharmaceutical Sciences Hoshi University

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