THE DISTRIBUTION OF RADIOACTIVE CARBON FROM [1-^<14>C]-GALACTOSAMINE IN MICE

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Tissue and organ distribution of radioactive carbon from ^<14>C-labeled galactosamine in the mouse was investigated by whole-body autoradiography and biochemical analysis. At 5 min after intraperitoneal injection of ^<14>C-galactosamine, high radioactivity was found in the abdominal cavity, liver and urine. The radioactivities of these organs were reduced by perchloric acid treatment of sections with the exception of urine. At 30 min after injection, the radioactivity of abdominal cavity became insubstantial, but the activity in the liver, small intestine and renal cortex was maintained at a high level which still remained 1 hr after injection, and was retained considerably after the acid treatment of sections. At 6 hr, the level of radioactivity became lower in many organs except for the liver, blood and renal cortex, at which time the activity of these organs was almost completely retained even after acid treatment of the sections. The comparative radioactive values in organs as estimated by a liquid scintillation counter were consistent with the values estimated by densitometry of autoradiographs. In animals at 5 and 30 min after injection, paper chromatography of the acid-soluble fractions of liver and kidney had a radioactive spot for galactosamine-1-phosphate but no spot for galactosamine could be detected.
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