Purification, Characterization, and Molecular Cloning of Tyrosinase from Pholiota nameko
スポンサーリンク
概要
- 論文の詳細を見る
Tyrosinase (monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, EC 1.14.18.1) was isolated from fruit bodies of Pholiota nameko and purified to homogeneity. The purified enzyme was a monomer with a molecular weight of 42,000 and contained 1.9 copper atoms per molecule. The N-terminal of the purified enzyme could not be detected by Edman degradation, probably due to blocking, while the C-terminal sequence of the enzyme was determined to be -Ala-Ser-Val-Phe-OH. The amino acid sequence deduced by cDNA cloning was made up of 625 amino acid residues and contained two putative copper-binding sites highly conserved in tyrosinases from various organisms. The C-terminal sequence of the purified enzyme did not correspond to that of the deduced sequence, but agreed with Ala384-Ser385-Val386-Phe387 in sequence. When the encoded protein was truncated at Phe387, the molecular weight of the residual protein was calculated to be approximately 42,000. These results suggest that P. nameko tyrosinase is expressed as a proenzyme followed by specific cleavage to produce a mature enzyme.
- 社団法人 日本農芸化学会の論文
- 2007-07-23
著者
-
GOTO Hideyuki
Department of Bone and Orthopaedics, Nagoya City University Graduate School of Medical Sciences
-
KAWANO Takeshi
Department of Electrical and Electronic Engineering, Toyohashi University of Technology
-
Goto Hideyuki
Department Of Anatomy Osaka Medical College
-
Suzuki Haruo
Division Of Biosciences Graduate School Of Fundamental Life Science Kitasato University
-
Suzuki Haruo
Division Of Bioscience Graduate School Of Basic Life Science Kitasato University
-
Kawamura Konishi
Division Of Biosciences Graduate School Of Fundamental Life Science Kitasato University
-
Kawano Takeshi
Department Of Biosciences School Of Science Kitasato University
-
Goto Hideyuki
Department Of Food Science Faculty Of Bioresources And Environmental Sciences Ishikawa Prefectural U
-
TSUJI Mariko
Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
-
HATANA Seiichi
Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
-
ASANUMA Masahiro
Department of Biosciences, School of Science, Kitasato University
-
KAKUTA Dai
Department of Biosciences, School of Science, Kitasato University
-
MUKOUYAMA Etsuko
Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
-
Kakuta Dai
Department Of Biosciences School Of Science Kitasato University
-
Tsuji Mariko
Division Of Biosciences Graduate School Of Fundamental Life Science Kitasato University
-
Hatana Seiichi
Division Of Biosciences Graduate School Of Fundamental Life Science Kitasato University
-
Asanuma Masahiro
Department Of Biosciences School Of Science Kitasato University
-
Mukouyama Etsuko
Division Of Biosciences Graduate School Of Fundamental Life Science Kitasato University
-
Kawamura-Konishi Yasuko
Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
-
SUZUKI Haruo
Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
関連論文
- Anthropometric, Lifestyle and Biomarker Assessment of Japanese Non-professional Ultra-marathon Runners
- THE DISTRIBUTION OF RADIOACTIVE CARBON FROM L-[U-^C] TYROSINE IN MICE
- THE DISTRIBUTION OF RADIOACTIVE CARBON FROM L-[SIDE CHAIN-3-^C]TRYPTOPHAN IN MICE
- DISTRIBUTION OF ^3H-CON A AND ^3H-WGA BINDING SITES IN MICE BY IN VITRO WHOLE-BODY AUTORADIOGRAPHY
- Signal Conditioning CMOS Circuits Integrated on Si (111) for Image-Recording Sensor of Neural Activity
- THE DISTRIBUTION OF RADIOACTIVE CARBON FROM [1-^C]-GALACTOSAMINE IN MICE
- THE DISTRIBUTION OF RADIOACTIVE CARBON FROM [U-^C] GLUCOSAMINE IN MICE
- Distribution of glucose-6-phosphatase in mice by in vitro whole-body autoradiography
- No V_H Somatic Hypermutation Was Detected in B-Cells of a Patient with Macroglobulinemia Due to Splenic Marginal Zone Lymphoma
- A novel variant of acute myelomonocytic leukemia carrying t(3;12)(q26;p13) with characteristics of 3q21q26 syndrome
- The effects of feeding a low protein or high protein diet on the glucose utilization in young mice brain.
- WHOLE-BODY FLUOROGRAPHY USING BLACK LIGHT FLUORESCENT TUBES
- Septic arthritis of the right ankle caused by Staphylococcus aureus infection in a rheumatoid arthritis patient treated with etanercept
- Realization of In Situ Doped n-Type and p-Type Si-Microprobe Array by Selective Vapor-Liquid-Solid (VLS) Growth Method
- Realization of n-type and p-type Si-Microprobe Array Using In-Situ Doping with Selective Vapor-Liquid-Solid (VLS) Growth Method
- Corynebacterium sp. U-96 Contains a Cluster of Genes of Enzymes for the Catabolism of Sarcosine to Pyruvate
- Multichannel 5 × 5-Site 3-Dimensional Si Microprobe Electrode Array for Neural Activity Recording System
- Kinetic Studies on the Role of Lys-171 and Lys-358 in the β Subunit of Sarcosine Oxidase from Corynebacterium sp. U-96
- Purification, Characterization, and Molecular Cloning of Tyrosinase from Pholiota nameko
- Sequencing and Expression of the L-Phenylalanine Oxidase Gene from Pseudomonas sp. P-501. Proteolytic Activation of the Proenzyme
- Effects of Dietary Concentration of Laminaran and Depolymerised Alginate on Rat Cecal Microflora and Plasma Lipids
- Fermentable Dietary Fiber in Dried Products of Brown Algae and Their Effects on Cecal Microflora and Levels of Plasma Lipid in Rats
- Kinetic Isotope Effect of the L-Phenylalanine Oxidase from Pseudomonas sp. P-501
- Channeling and conformational changes in the heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96
- DISTRIBUTION AND METABOLIC FATE OF RADIOACTIVE CARBON FROM L-[U-^C]ARGININE ADMINISTERED INTO MICE
- Realization of In Situ Doped n-Type and p-Type Si-Microprobe Array by Selective Vapor-Liquid-Solid (VLS) Growth Method
- Multichannel $5 \times 5$-Site 3-Dimensional Si Microprobe Electrode Array for Neural Activity Recording System
- Glucose-6-phosphatase Activity in Rat Bone
- Deuterium kinetic isotope effects in heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96 : the anionic form of the substrate in the enzyme-substrate complex is a reactive species