THE DISTRIBUTION OF RADIOACTIVE CARBON FROM [U-^<14>C] GLUCOSAMINE IN MICE
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概要
- 論文の詳細を見る
Tissue and organ distribution of radioactive carbon from ^<14>C-labeled glucosamine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intraperitoneally with D-[U-^<14>C]-glucosamine were sacrificed at various intervals. Examination of auto-radiographs disclosed that the injected ^<14>C-glucosamine was almost completely absorbed from the abdominal cavity 30 min after injection. At 5 min after injection, the highest radioactivity was observed in the liver, but the activity in the blood and brain was low. The radioactivity in these organs was made negligible by perchloric acid treatment of sections. At 30 min after injection, high radioactivity was detected in the liver, small intestine, preputial gland and urine. At 3 hr after injection, the liver, blood and cortex of the kidney had high radioactivity. The comparative values among radioactivity in various organs estimated by a liquid scintillation counter were consistent with those obtained from whole-body autoradiographs. At 30 min after injection, an increase of radioactivity in the acid-insoluble fractions was observed in all organs examined. In animals at 5 and 30 min after injection, paper chromatography of the acid-soluble fractions disclosed radioactive spots for glucosamine and N-acetyl-glucosamine in the blood, N-acetylglucosamine and glucose in the kidney and glucosamine, glucuronic acid and glucose in the liver. By gel electrophoresis, a radioactive protein with molecular weight of 90,000 was detected in the acid-insoluble fraction of kidney at 30 min after injection.
- 日本組織細胞化学会の論文
著者
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Kihara Takashi
Department Of Cardiovascular Medicine
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Kihara T
Osaka Medical Coll. Takatsuki Jpn
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Kihara T
Department Of Anatomy Osaka Medical College
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GOTO Hideyuki
Department of Bone and Orthopaedics, Nagoya City University Graduate School of Medical Sciences
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Kihara Takashi
2nd Department Of Anatomy Osaka Medical College
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Kihara Takashi
Department Of Anatomy Osaka Medical College
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WATANABE Masahito
Department of Physics, Gakusyuin University
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SHIMONO RYOICHI
Department of Anatomy, Osaka Medical College
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Goto Hideyuki
Department Of Anatomy Osaka Medical College
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Watanabe M
Department Of Anatomy Osaka Medical College
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Goto H
Osaka Medical Coll. Osaka
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Shimono Ryoichi
Department Of Anatomy Osaka Medical College
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Shimono Ryoichi
Department Of Anatomy Kagawa Medical School
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Watanabe Masahito
Department Of Anatomy And Cell Biology Osaka Medical College
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WATANABE Masahito
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University
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