DISTRIBUTION AND METABOLIC FATE OF RADIOACTIVE CARBON FROM L-[U-^<14>C]ARGININE ADMINISTERED INTO MICE
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概要
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Tissue and organ distribution of radioactive carbon from ^<14>C-labeled arginine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intravenously with L-[U-^<14>C]arginine were sacrificed at various intervals. Examination of autoradiographs disclosed that the injected ^<14>C-arginine was rapidly taken up from the blood by the organs. The radioactivity in the pancreas was the highest throughout the intervals after injection in this investigation. The general pattern of the autoradiographs obtained after injection of radioactive arginine had a resemblance to those reported about other amino acids. The comparative values among radioactivity in various organs estimated by a liquid scintillation counter were consistent with those obtained from whole-body autoradiographs. Radioactivity in the acid-insoluble fractions was increased with time in all organs examined. High-performance liquid chromatography of the acid-soluble fractions disclosed that several radioactive substances were detected in the examined organs soon after injection and that the amounts of the radioactive arginine and its metabolites and/or their molar ratios were different among the organs.
- 日本組織細胞化学会の論文
著者
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Goto Hideyuki
Department Of Anatomy Osaka Medical College
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Goto H
Department Of Anatomy Osaka Medical College
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