TISSUE DISTRIBUTION AND METABOLIC FATE OF ^<35>S FROM ^<35>S-TAURINE IN PREGNANT MICE BY WHOLE-BODY AUTORADIOGRAPHIC AND BIOCHEMICAL STUDY
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概要
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Tissue distribution and metabolic fate of ^<35>S from ^<35>S-taurine in pregnant mice were studied by whole-body autoradiography and biochemical analysis. Whole-body autoradiography was performed at 30 min, 3 and 6 hr after intravenous injection of ^<35>S-taurinc. In the maternal body, high densities were observed in the kidney, liver, bile and digestive tract, while low densities were in the blood and brain. The placenta showed a relatively high density, but the fetus was low density. As for the biochemical analysis, the mice were decapitated at the same intervals after injection as those for autoradiography and various tissues were removed. These tissues were fractionated into 6% perchloric acid-soluble, -insoluble and lipid fractions. Total radioactivities (cpm) per wet weight (g) were high in the liver, small intestine and kidney and low in the brain, blood and fetus. These data were consistent with those of whole-body autoradiographs. The greatest part (over 98%) of total radioactivities in all tissues was incorporated into the acid-soluble fraction. The acid-soluble fraction of each tissue was analyzed by thin-layer chromatography. The radioactive spots were examined for taurine and taurocholic acid in the bile, but only for taurine in all other tissues.
- 日本組織細胞化学会の論文
著者
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SHIMONO RYOICHI
Department of Anatomy, Osaka Medical College
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SHIMADA Masahisa
Departments of Anatomy, Osaka Medical College
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Shimada Masahisa
Department Of Anatomy Kagawa Medical School
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Shimono Ryoichi
Department Of Anatomy Kagawa Medical School
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