TISSUE DISTRIBUTION AND METABOLISM OF RADIOACTIVE SULFUR AMINO ACIDS ([1-^<14>C]CYSTINE, [^<35>S]CYSTEINE) IN PREGNANT MICE; A WHOLE-BODY AUTORADIOGRAPHY AND ITS BIOCHEMICAL ANALYSIS
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概要
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Tissue distribution and metabolic fate of ^<14>C from [1-^<14>C]cystine and ^<35>S from [^<35>S]cysteine, both precursors of taurine, in various tissues and organs of pregnant mice were studied by whole-body autoradiographic and biochemical analysis. Autoradiography of whole-body cryosection was performed at 30 min, 3 and 6 hr after i.v. injection of radioactive sulfur amino acids ([1-^<14>C]cystine and [^<35>S]cysteine). In the maternal body, the highest optical density (00) was observed in the pancreas and then the kidney, small and large intestines, stomach, Harderian gland, liver, salivary gland and mammary gland followed. Low ODs were observed in the brain and skeletal muscle. ODs of ^<14>C and ^<35>S in the placenta were relatively high. In the fetus, high ODs of ^<14>C were observedin the lens and ^<35>S in the cartilage and lens. In the biochemical analysis, the mice were decapitated at the same intervals after injection as those for autoradiography and various tissues were removed. These tissues were fractionated into 6% perchloric acid-soluble, -insoluble and lipid fractions. Total radioactivities (cpm/g wet weight) of ^<14>C and ^<35>S were highin the kidney, pancreas, Harderian gland and stomach, and low in the brain,skeletal muscle and fetus. The liver and bile showed high radioactivities in ^<35>S,but low radioactivities in ^<14>C. These data were in good agreement with those of whole-body autoradiographs. The radioactivities incorporated into the acid-in-soluble fraction were high in the pancreas, stomach, large intestine and salivary gland, but low in the liver, kidney and skeletal muscle after injection of [1-^<14>C]cystine and [^<35>S]cysteine. The acid-soluble fractions of the liver, kidney, pancreas and small intestine were analyzed by thin-layer chromatography. The radioactive spots for cysteine, cysteine sulfinic acid (CSA) and taurine were detected, but the radioactive level and its change with time in each radioactive spot were different among the organs.
- 日本組織細胞化学会の論文
著者
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SHIMONO RYOICHI
Department of Anatomy, Osaka Medical College
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SHIMADA Masahisa
Departments of Anatomy, Osaka Medical College
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Shimada M
Department Of Anatomy Osaka Medical College
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Shimada Masahisa
Department Of Anatomy Kagawa Medical School
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YAMAGUCHI KENJI
Division of Maternal and Child Nutrition, The National Institute of Nutrition
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HOSOKAWA YU
Division of Maternal and Child Nutrition, The National Institute of Nutrition
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Hosokawa Yu
Division Of Maternal And Child Nutrition The National Institute Of Nutrition
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Shimono R
Department Of Anatomy Kagawa Medical School
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Shimono Ryoichi
Department Of Anatomy Kagawa Medical School
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Yamaguchi Kenji
Division Of Maternal And Child Nutrition The National Institute Of Nutrition
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HOSOKAWA Yu
Division of Maternal & Child Nutrition, The National Institute of Health and Nutrition
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