THE DISTRIBUTION OF [U-^<14>C]FRUCTOSE IN THE MICE STUDIED BY WHOLE-BODY AUTORADIOGRAPHY
スポンサーリンク
概要
- 論文の詳細を見る
Tissue and organ distribution of ^<14>C from ^<14>C-labeled fructose in the mouse was studied by whole-body autoradiography. The mice were injected intraperitoneally with 12 uCi of D-[U-^<14>C]fructose and killed after different intervals (5, 30 min, 1, 3, 6 and 24 hr). Autoradiography of the whole-body sections (untreated and treated with ice-cold 6% HC1O_4) and densitometry were carried out. Untreated autoradiographs: At 5 min after injection, a large amount of the injected fructose still remained in the abdominal cavity. Highest autoradiographic densities at time were in the kidney, liver and blood. The large and small intestinal mucosa showed relatively high density. At 30 min, the highest densities occured in the large intestinal mucosa, Harderian gland and kidney medulla, while the abdominal cavity did not show any significant radioactivity. At 1 hr, the highest density occurred in the large intestinal mucosa, while a relatively high density was found in the small intestinal mucosa, kidney, sublingual and Harderian glands, and pancreas. At 3 hr, the sublingual and submandibular glands attained the highest density. In the other organs, however, the densities at this time were lower than those at 1 hr. At 6 and 24 hr, the densities in all tissues and organs except for the brown fat decreased compared with those at 3 hr. Treated autoradiographs: Incorporation of ^<14>C from [U-^<14>C]fructose into acid-insoluble substances was high for all glands observed, but low for the blood, liver, brain, muscle, and lung.
- 日本組織細胞化学会の論文
著者
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Kihara Takashi
Department Of Cardiovascular Medicine
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Kihara T
Osaka Medical Coll. Takatsuki Jpn
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Kihara T
Department Of Anatomy Osaka Medical College
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Kihara Takashi
2nd Department Of Anatomy Osaka Medical College
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Kihara Takashi
Department Of Anatomy Osaka Medical College
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WATANABE Masahito
Department of Physics, Gakusyuin University
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SHIMONO RYOICHI
Department of Anatomy, Osaka Medical College
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Watanabe M
Department Of Anatomy Osaka Medical College
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Shimono Ryoichi
Department Of Anatomy Osaka Medical College
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Shimono Ryoichi
Department Of Anatomy Kagawa Medical School
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Watanabe Masahito
Department Of Anatomy And Cell Biology Osaka Medical College
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WATANABE Masahito
Department of Agricultural and Biological Chemistry, College of Bioresource Sciences, Nihon University
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