Induction of Apoptosis in Mice with B16 Melanoma by Treatment with the Antimicrotubule Agent TZT-1027
スポンサーリンク
概要
- 論文の詳細を見る
TZT-1027, an inhibitor of tubulin polymerization, is a derivative of dolastatin 10 isolated from the Indian Ocean sea hare Dolabella auricularia. TZT-1027 has good anticancer activity. We investigated the effect on B16 melanoma of TZT-1027 in mice histopathologically and by agarose gel electrophoresis. At 1 mg/kg of TZT-1027, there was a good correlation between the number of mitosis-like cells and apoptotic bodies. In mitosis-like cells, the nuclear membrane had disappeared, and the chromosomes had morphological features typical of mitosis. However, not all cells in mitosis had proliferated. In contrast, at 2 mg/kg of TZT-1027, many apoptotic bodies were detected soon after treatment, but these were not involved in the dynamic changes to mitosis-like cells. Furthermore, TUNEL-positive cells were clearly visible at 1 hr after treatment and the nuclei of these cells showed chromatin margination, cap-shape chromatin clamping, and nuclear condensation on electron microscopy. Therefore, we speculated that the effect on B16 melanoma cells of TZT-1027 differed with the treatment dose, and a dose of 2mg/kg induces apoptosis in tumor cells by a direct mechanism.
- 日本組織細胞化学会の論文
著者
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Mikami T
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Tagawa Masashi
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Watanabe Junichi
Aska Pharmaceutical Co. Ltd. Research & Development Division
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Kobayashi Masatoshi
Department Of Anatomy & Histology Kurume University School Of Medicine
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Kobayashi Makio
Dept. Of Pathology Tokyo Women's Medical University
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NAKAYAMA Takaharu
Safety Res. Dept., Teikoku Hormone Mfg. Co., Ltd., of Kanagawa Pref.
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Kobayashi M
Departments Of Pathology Tokyo Women
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MURAKOSHI Masanori
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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Ikeda Rie
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Murakoshi M
(present Address)products Strategy Dcpartment Pharma Business Strategy Division Teikoku Hormone Mfg.
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Watanabe Jun
Department Of Pathology School Of Medicine Kitasato University
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Ohtani Masashi
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Murakoshi Masanori
Safety Res. Dept. Teikoku Hormone Mfg Co. Ltd.
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Mikami T
Department Of Drug Safety Research Eisai Kawashima Research Laboratories Eisai Co. Ltd.
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Nakayama Takaharu
Medicinal Research Department Teikoku Hormone Mfg. Co. Ltd.
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Nakayama Takaharu
Safety Res. Dept. Teikoku Hormone Mfg. Co. Ltd. Of Kanagawa Pref.
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NATSUME Tsugitaka
Pharmacological Research Department, Teikoku Hormone Mfg. Co., Ltd.
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KOBAYASHI Motohiro
Pharmacological Research Department, Teikoku Hormone Mfg. Co., Ltd.
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MIKAMI Takashi
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd.
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Watanabe Junichi
Pharmacological Research Department, Teikoku Hormone Mfg, Co.Ltd.
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Natsume Tsugitaka
Teikoku Hormone Mig. Co. Ltd.
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Kobayashi M
Aska Pharmaceutical Co. Ltd. Research & Development Division
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Inada Rie
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Ikada Rie
Safety Research Department Teikoku Hormone Mfg. Co. Ltd.
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Nakayama T
Aska Pharmaceutical Co. Ltd. Research & Development Division
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