口腔微生物の生態学的研究 : 3. 定量的簡易グラム染色法

概要

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A newquantitative method of gram stainmodifying after Hucker was devised and established. It is also rapid and simple as follows. 1. Prepare a thin film of the material to be examined and dry in air. 2. Fix with absolute alcohol for 60 sec after being flamed. 3. Stain with 0.2% crystal violet solution for 60 sec. 4. Wash with water. 5. Stain with Lugol solution for 60 sec. 6. Wash gently with tap water. 7. Decolorize with the mixture of 70ml of 95% ethanol and 30ml of acetone for 90 sec. 8. Wash with water and dry in air. 9. Decolorize with 5 ml of 0.3% HCl in 70% ethanol. 10. The latter decolorizing solution is measured for absorbence at 580 mμ. This method showed a clear linear relationship between the concentration of bacteria and the extinction of crystal violet at 580 mμ.
九州歯科学会の論文
1973-09-30