誘導マクロファージの機能に関する研究 : I 報マクロファージの Ia 抗原の測定法について

概要

論文の詳細を見る
In induced peritoneal macrophages, the quantity of Ia antigen was measured by monitoring absorbance using enzyme-linked immunosorbent assay (ELISA). The number of Ia positive cells were counted simultaneously under microscope. Results obtained were as follows : 1. An optimum time for enzyme reaction in ELISA was 40 min using 4×10^4 cells as a sample. 2. Absorbance in ELISA correlated closely with the number of Ia positive cells. 3. It was shown that an increase in absorbance was brought about by an increase in the number of Ia positive cells and not by an increase in Ia quantity in the surface each cell. These results showed that ELISA was a useful method for the study of quantifying cell surface antigens like Ia antigen.
九州歯科学会の論文
1986-12-25