歯周疾患と Interleukin-1 (IL-1) に関する研究 : 第 1 報ヒト歯肉組織中の IL-1 測定法について

概要

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It is suggested that Interleukin-1 (IL-1) is associated with the destruction and restoration of periodontal tissues. The purpose of this paper was to establish an assay system of IL-1 in human gingival tissue. We quantitatively measured IL-1 in the extract from human gingival tissue by an enzyme-linked immunosorbent assay (ELISA). The assay procedure is composed of 5 steps : 1) incubation of experimental gingival extract diluted with PBS from 1 : 2500 to 1 : 1000000 for 2h at 37℃ ; 2) washing with PBS containing 0.1% Tween 20 three times, followed by coating with Block Ace at a 1 : 4 dilution with distilled water for 1h at 37℃ ; 3) washing three times, followed by incubation with rabbit anti-human IL-1 at a 1 : 1000 dilution with PBS for 2h at 37℃ ; 4) washing three times, followed by incubation with peroxidase conjugated anti-rabbit IgG at a 1 : 1000 dilution with PBS for 2h at 37℃ ; and 5) washing three times, followed by addition of the substrate solution and incubation for 1h at 37℃. The absorbance at 405 nm was measured using EIA-READER (BIO-RAD). The absorbance increased linearly in the range of 0.2-0.9 as concentration-dependent manner in each extract. The result showed that ELISA was a useful method for the quantification of IL-1 in human gingival tissue. A close correlation between the amount of IL-1 and alveolar bone loss was found.
九州歯科学会の論文
1988-12-25