誘導マクロファージの Ia 抗原に関する研究 : 第 1 報 T 細胞との混合培養による Ia 抗原量の変化について

概要

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Ia antigen of induced macrophage (MφIa) was measured using the method of enzyme-linked immunosorvent assay (ELISA). The macrophages were cultured in vitro with spleen T cells (Ts-nor), thymus T cells (Tt-nor) and their supernates. The macrophages were also cultured with T cells (Ts-stim and Tt-stim), which were stimulated with Concanavalin A (Con A), and their supernates. The results were as follows ; 1. The quantity of MφIa increased when the macrophages were cultured with Ts-nor, Tt-nor, Ts-stim, Tt-stim and their supernates. 2. It appeared that the quantity of MφIa increased when the macrophages were cultured with Con A-stimulated T cells. 3. Ts made the increase of the quantity of MφIa higher than Tt. Ts seemed to be more sensitive than Tt concerning the stimulation by Con A. 4. The macrophages, which were cultured with T cells indirectly using the membrane filter, had more MφIa than the macrophages which were cultured with T cells directly. These results showed that T cells matured and made the increase of MφIa during the 24hr-culture. Concerning their ability to increase the quantity of MφIa, Ts was higher than Tt, and this ability was strengthened by Con A-stimulation. On the other hand, it was suggested that MφIa dissappeared or changed rapidly after Ia antigen was received by T cell. The indirect culture used here was useful in the study on the cell-cell interaction.
九州歯科学会の論文
1987-04-25