水溶性ポルフィリンによる微量金属イオンのけい光分析

概要

論文の詳細を見る
Water-soluble porphyrins, α,β,γ,δ-tetraphenyl-porphinetrisulfonate (TPPS) tetra (4N-methylpyridyl)-porphine and tetra (4-carboxyphenyl)porphine, and zinc(II)- and magnesium(II)-complexes of these porphines have intense red fluorescence spectra at wavelength range of 600 nm to 700 nm. And copper (II), palladium(II), cobalt(II) and lead(II) quantitatively quenched the fluorescence of these porphines at a room temperature by complexation. Based on these findings, the possible analytical applications of the luminescence phenomena to the determination of Pd(II), Co(II), Pb(II) and Cu(II) were discussed, and the quenching-spectrofluorometric determination for ppb level of copper (II) with TPPS was demonstrated. A recommended procedure: take an about 40 cm^3 solution containing (0,3〜3)μg of copper(II) into a 100cm^3 beaker, add 2.5 cm^2 of 1 mol dm^<-3> acetate buffer solution (pH 4.0) and 5 cm^3 of 1 × 10^<-5> mol dm^<-3> standard TPPS solution. Heat the mixture to boil for one min and allow to cool to room temperature. Then, adjust pH to 2.5 with 1.5 cm^3 of 1 mol dm^<-3> chloroacetic acid solution, dilute to 50 cm^3 with water and measure the fluorescence intensity of H_4tpps^<2+> at 657 nm (excitation wavelength ; 434nm) with,a 1 cm quartz cell. 1000μg of Ba^<2+>, Cd^<2+>, Cr^<3+>, Ga^<3+>, Mg^<2+>, Mo^<6+>, Na^+, Pb^<2+>, VO_3-and W^<6+>, 100μg of Ag^+, Hg^<2+> and Sn^<2+>, and 10μg of Al^<3+>, Co^<2+>, Fe^<2+>, Fe^<3+>, In^<3+>, Mn^<2+> and Ni^<2+> did not interfere with the determination. However, 10μg of Pd^<2+> and Zn^<2+> interfered. The calibration curve was found to be linear in the range of 0.3 μg/50 cm^3 to 3.18μg/50 cm^3. The coefficient of variation was 1.69% for 1.27 μg/50 cm^3 of Cu^<2+> (8 determinations).
社団法人日本分析化学会の論文
1979-07-05

水溶性ポルフィリンによる微量金属イオンのけい光分析

スポンサーリンク

概要

著者

関連論文

スポンサーリンク