チタン(IV)との呈色反応を利用するデスフェリオキサミンBの簡易吸光光度定量

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Based on the color reaction with titanium (IV), a simple spectrophotometric method for the determination of 10^<-5> mol dm^<-3> levels of DFB was developed. The recommended procedure was carried out as follows : an aliquot of a sample solution (15 cm^3) containing less than 4.4 mg of Desferrioxamine B mesylate was taken into a 25 cm^3 volumetric flask, and 4 cm^3 of a 1 mol dm^<-3> hydrochloric acid solution and 5 cm^3 of a 1×10^<-3> mol dm^<-3> titanium (IV) solution were added and the mixture was diluted to the mark with water. After 5 min, the absorbance at 356 nm was measured against water. The calibration curve was linear over the DFB concentration range 2×10^<-5>〜2×10^<-4> mol dm^<-3>. The relative standard deviation was 0.65% for [DFB]_T=1×10^<-4> mol dm^<-3> (12 determinations). The detection limit was 1.31×10^<-6> mol dm^<-3> (S/N=2). For the determination of 4×10^<-5> mol dm^<-3> DFB, diverse ions were tolerated up to 1000-fold molar excess of NO_3^-, Cl^-, ClO_4^-, SO_4^<2->, K (I) and Na (I) ; they were tolerated up to a 100-fold molar excess of EDTA, glycine, Al (III), Co (II), Cu (II), Pb (II) and Zn (II), respectively. For the determination of 4×10^<-5> mol dm^<-3> DFB, equal amounts of Fe (III), V (V) and Mo (VI) interfered ; however, a 50-fold molar excess of Fe (III) was tolerated with the use of [HNO_3]_T=1.5 mol dm^<-3> instead of [HCl]_T=0.16 mol dm^<-3>.
社団法人日本分析化学会の論文
1991-10-05