亜硫酸塩-過酸化水素系自己触媒反応を用いる過酸化水素の目視マイクロプレート分析法

概要

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The oxidation reaction from sulfite ion to sulfate ion with hydrogen peroxide is an autocatalytic reaction, in which a proton (H^+) acts not only as the reaction product, but also as the reactant. This reaction provides the characteristic S-shape pH vs. time curve, which contains the inflection time and the dynamic pH change at the end point. The threshold of the concentration of hydrogen peroxide exists in the presence of the progress of this reaction, and the value of the threshold is determined by the ratio of the concentration for several concentrations of a sulfite ion. Furthermore, the pH change at the end point can be visualized using a pH indicator, such as bromothymol blue. Based on these findings, using this reaction for the microplate, the visual determination of hydrogen peroxide by counting the discoloring color number has been developed. By the proposed method, 2.5×10^<-3>〜1.0mol dm^<-3> of hydrogen peroxide could be visibly determined. Moreover, the influence of the temperature was examined. As a result, the analytical method was able to be used within a wide range of temperatures.
社団法人日本分析化学会の論文
2008-02-05