水溶性ポルフィリン金属錯体の高速液体クロマトグラフィーを利用する多成分金属イオンの同時定量

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The properties of porphyrin compounds as chelating reagents in high performance liquid chromatography (HPLC) were studied and simultaneous spectrophotometric determination of (10^<-8>〜10^<-9>)g/cm^3 levels of Cu^<2+>, Zn^<2+>, and Pd^<2+> by HPLC with α,β,γ,δ-tetrakis (l-methylpyridinium-4-yl) porphine [TMPyP] has been developed. Recommended procedures: take a sample solution containing up to 2.O μg of metal ions into a 50cm^3 beaker, add 1cm^3 of 1×1O^<-4> mol dm^<-3> TMPyP solution and 2cm^3 of pH buffer solution (pH5.O〜7.O). Heat the mixture to boil for 30 min and allow to cool to room temperaturc and then dilute to 25 cm^3 with water. Inject O.1cm^3 of the mixture in to HPLC {mobile phase: 50/50%(v/v) H_20-CH_3COOH solution containing O.35 mol dm^<-3> of sodium dodecylsulfate, flow rate 1.0 cm^3/min} and measure the chromatogram at the detecting wavelength, 430 nm, and the absorbance unit full-scale, O.16. The molar absorption coefficent (ε,cm^<-1> mol^<-1>dm^3) of each complex at 430 nm was 1.88×10^5,1.88×10^5, and 0.64x10^5 for copper, zinc, and palladium, respectively. The calibration curves of these metal ions with the peak height of the chromatogram were found to be linear in the range of 0〜0.08μg/cm^3, and the coefficient of variation (5 determinations) at a center of the above range was 0.95% for Cu^<2+>, 2.02% for Zn^<2+>, and 3.57% for Pd^<2+>.
社団法人日本分析化学会の論文
1983-01-05