醤油有機酸に関する研究(第1報) : 醤油有機酸の定量法について

概要

論文の詳細を見る
The authors found that it is possible to determine organic acids of "Kiage Syoyu" and "Aminosan-eki" separately by the partition chromatography according to C. MARVEL's method after extraction of them with ether.However, it has been necessary to repeat the partition chromatography according to BULLEN's method, since the separation between lactic acid and succinic acid was not completed by C. MARVEL's method.During the extraction of organic acids with ether, lactic acid in samples polymerized to form lactyl-lactic acid and lactyl-lactyl-lactic acid, of which peak effluent volumes were similar to those of pyruvic acid and acetic acid respectively, and consequently induced the errors in the determination.By the partition chromatography, the paper chromatography and characteristic reaction of each organic acid, it was found that the organic acid in "Kiage Syoyu" consists of butyric, isobutyric, levulinic, acetic, pyruvic, formic, α-ketoglutaric, lactic, succinic, pyroglutamic, glycollic, malic and citric acids and an unidentified acid. The composition of organic acid in "Aminosan-eki" was similar to that in "Kiage Syoyu", though there was some difference between them in the amount of each organic acid.
社団法人日本生物工学会の論文
1959-03-15