微生物の動特性に関する研究 : Azotobacter vinelandiiの回分, 連続培養における2,3の実験

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After confirming in the preliminary experiment that a specific strain of Azotobacter vinelandii used here proliferated favorably and exhibited an acceptable degree of nitrogen-fixing capacity in Burk's medium, both types of batch and continuous culture of the bacterium were examined. The Warburg technique was employed to assess Q_<o2> and Q<co2> values. The bacterium was found to be markedly active in its respiration : the Q<o2> and Q<co2> values were about fifteen times as high as those of other species of bacteria.The eventual objective of this study which will continue in a series, is to find out any rule governing the dynamic behavior of the bacterium in meeting with abrupt changes of environmental conditions from one to another in continuous culture. As a first approach to this goal, steady state in continuous culture of the strain was suspended artificially by changing step-wise a dilution rate from one level to another. The change of cell mass and glucose concentrations with time in the culture medium was determined experimentally; an Analog computer was then used to assess the change of specific growth rate and yield factor of the strain observed during unsteady state which followed the step-change of the dilution rate. Although much more work remains to be done, use of the Analog computer demonstrated here in this account is considered to be worthy of note.
社団法人日本生物工学会の論文