熱殺菌に関する研究 : (第2報)微生物細胞の物性値について : (その1 密度)

概要

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The density of microbial cells was determined by applying the law of additivity of mass to the cell suspension, as shown in Eq.(1). First, the value of cellular volume fraction in a specific microbial suspension was determined using the "Inulin Space Method".Secondly, the density of the suspension of microbial cells was determined by selecting a specific mixture of xylene and o-dichlorobenzene, in which a droplet of the suspension neither floated nor settled.This procedure can also be used for measuring the density of various species of micro-bes. The volume fraction of an actinomycete, for instance, cannot be evaluated microscopically, and in addition, the density of a pasty and thick cell suspension cannot be determined picnometrically; both of these facts do not lend themselves to a proper determination of density of cells in question.The values of density thus obtained using bacterium, bacterial spore, yeast, actinomycete and fungus ranged from 1.05 to 1.24g/cm^3 depending on the species, as shown in Table 2.
社団法人日本生物工学会の論文
1966-07-25