Bacillus caldolyticusの連続培養におけるα-アミラーゼ生産活性の細胞間分布について

概要

論文の詳細を見る
Mutant cells with constitutive production of α-amylase had grown predominantly in the continuous culture of Bacillus caldolyticus strain SP. With 36 cell types isolated as single colonies from the chemostat culture, the α-amylase production of each cell type was measured in a batch culture using a medium without maltose (the inducer). The cell population was divided in two groups; one, with higher α-amylase production (strain MH); the other, lower (stain ML). The fraction of strain MH cells among the total viable cells changed depending on the dilution rate. The fraction was largest at an opimum dilution rate giving the highest α-amylase activity of the culture. Similar results were obtained regarding the dilution rate effects on α-amylase activity when the cells of the strain ML or MH were used as a seed strain. In the continuous culture of the strain MH, it changed to the strain ML cells reversibly. However the transition of the ML cells or MH cells to SP cells was not observed. Those findings suggest that the population change for strain SP to strain ML and strain MH may be as follows; [chemical formula]
社団法人日本生物工学会の論文
1989-03-25