メタン酸化細菌の同定と培養条件の検討

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A methane-oxidizing bacterium, strain 2-19,isolated from paddy soil in the methane gas leaking area was investigated with respect to characterization and culture conditions. Strain 2-19 was a gram-negative, non-motile, non-sporeforming, catalase-positive, oxidase-negative, strict aerobic bacterium which grew only on methane and methanol. It showed rod-shaped cells throughout whole cultural period and a resting stage was not observed. Strain 2-19 was a different species from M. methanica and M. methanooxidans. It should belong to Methylomonas according to he classification of Whittenbury, Phillipus and Wikinson, but it did not fall into any species described as Methylomonas. Besides all methane-oxidizing bacteria which they isolated gave positive reaction on the oxidase test, but strain 2-19 gave negative reaction. Strain 2-19 is apparently distinct from other methane-oxidizing bacteria and the differences are great enough to justify a separate species. The authors propose the name Methanomonas margaritae sp. n. Takeda, Motomatsu, Hachiya, Fukuoka et Takahara 1974 for the new methane-oxidizing bacterium.M. margaritae required copper as a micronutrient. The decrease of growth rate at he initial stage was due to deficiency of copper ion. The maximum specific growth rate (0.15hr^<-1>) was obtained when an ammonium salt was used as the nitrogen source and the methane was mixed with oxygen and nitrogen in a ratio of 60 : 15 : 25. When M. margaritae was cultured in the medium supplemented with copper sulfate, the dimension of cells was 1.4×3.0μm and the ratio of protein to dry cell was 63%. In copper deficient medium, the former was 1.2×2.0μm and the latter 43%.
社団法人日本生物工学会の論文
1974-11-25

メタン酸化細菌の同定と培養条件の検討

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