Rhizopus属の生成するセルラーゼについて : (第1報)培地組成と力価の検定

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The purpose of this study was to find the distribution of cellulase producing activity and to select some high power strains in the Rhizopus genus.This report describes the medium composition for selection and assay methods for celullase.DPC medium of the following compsition was adopted for selection, using a DP medium as the basal medium, since it was found to be suitable for the growth of Amylomold, DPC medium : CMC 0.5%, Dextrin 1.0%, Peptone 1.5%, KH_2PO_40.5%, MgSO_47H_2O 0.25%, initial pH 4.3.For assay in the activity of cellulase, breakdown of filter paper and hydrolysis of CMC were adopted. The former way was according to Toyama's method and the latter was examined in the present work.The optimal conditions were set at pH 5.0,at 50℃, for 60 minutes. Measuring conditions for celullase activity were the same as that for amylase, making this a convenient method.
社団法人日本生物工学会の論文
1964-06-15