Methanomonas margaritaeの菌体生産について

概要

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Experiments were directed towards the production of high biomass concentrations in cultures of Methanomonas margaritae. To obtain bacterial cells with high protein content steadily and efficiently, some aspects of growth of the organisms relating with cultural conditions were studied.Copper ions improved growth of the organism on methane. The addition of 0.17 mg copper ions supported well the cell production of 1 g dry weight. When the organism was grown in copper-deficient medium, the increase of dry cell weight shifted down at the beginning of growth as compared with the copper-sufficient culture, but the rate of increase in viable number decreased a few hours later. The cells of the former were smaller in size and lower in protein content than the latter.Optimum gas composition for the growth was 10-15% O_2. 60-80% CH_4. A high oxygen partial pressure of more than 20% inhibited the growth.The carbon balance in the culture of M. margaritae growing on methane was investigated under the conditions mentioned above. Cell-C was twice as high as extracelular products-C and protein content was about 60%. Yield of bacterial cells growing on methane was 63%.Extracellular products were polysaccharide, pyruvic acid and amino acids. From these results, the following equation for growth was obtained.[numerical formula].
公益社団法人日本生物工学会の論文
1977-11-25