Bacillus polymyxa 1変株の生成するBacterial Polysaccharideの研究(第4報) : Polysaccharideの精製

概要

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In the present work, further purification of the Ca-free material from the crude Ca-polysaccharide of B. polymyxa var. lactoviscosus has been accomplished.Deproteining was performed, after removal of calcium with dil. HCl (pH 2.2) and dialysis against distilled water, by extracting with chloroform-isoamyl alcohol, according to SEVAGE'S method (1934).This refined polysaccharide is essentially nitrogen- and ash-free and yields approximately 97.8% reducing sugar, as glucose, on hydrolysis.Since copper-polysaccharide complex was formed from the Ca-free polysaccaride in FEHLING'S reagent (CuO; 15.16%), and the viscosity of Ca-free material was much lower than that of Ca-polysaccharide, one would be obtained some informations concerning the pattern of combination between calcium and polysaccharide.
社団法人日本生物工学会の論文
1954-08-15