Purification, Properties, and N-Terminal Amino Acid Sequences of Guar Gum-degrading Enzyme from Bacillus circulans K-1
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概要
- 論文の詳細を見る
A guar gum-degrading enzyme of the newly isolated Bacillus circulans K-1 was purified to an electrophoretically homogeneous state. The molecular weight of the purified enzyme was 62,000 by SDS-PAGE. The purified enzyme was separated into at least six isozymes by isoelectric focusing and the pI of these isozymes were 5.4, 5.5, 5.6, 5.8, 6.0, and 6.2, respectively. The N-terminal amino acid sequences of the typical three of these proteins were all the same, Ala-Ser-Gly-Phe-Tyr-Val-Ser-Gly-Thr-Lys-Leu-Leu-Asp-Ala-Thr-Gly-Gln-Pro-Phe-Val-Met-Arg. The enzyme was most active at pH 6.9 and at 64℃. The enzyme was activated slightly by Al^<3+> and inhibited strongly by Sn^<2+> and Zn^<2+>, N-bromosuccinimide, 2-mer-captoethanol, and ethylenediamine-tetraacetic acid.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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Yoshida Seiji
Research Institute Of Technology Konoike Construction Co. Ltd.
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Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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SAKO Yoshihiko
Laboratory of Marine Microbiology, Department of Applied Bioscience, Graduate School of Agriculture,
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UCHIDA Aritsune
Laboratory of Marine Microbiology, Department of Applied Bioscience, Graduate School of Agriculture,
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Sako Y
Graduate School Of Agriculture Kyoto University
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Sako Yoshihiko
Laboratory Of Microbiology Department Of Fisheries Faculty Of Agriculture Kyoto University
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Sako Yoshihiko
Laboratory Of Marine Microbiology Division Of Applied Biosciences Graduate School Of Agriculture Kyo
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Uchida A
Graduate School Of Agriculture Kyoto University
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Uchida Aritsune
Laboratory Of Marine Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Ky
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Sako Yoshihiko
Laboratory Of Marine Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Ky
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