Phylogenetic Analysis by 16S rRNA Gene Sequencing of Obligate Oligotrophs Isolated from the Northern Basin of Lake Biwa (Mesotrophic Lake)

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概要

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• We determined the number of total oligotrophs (obligate and facultative) in the northern basin of Lake Biwa (mesotrophic lake) on May 9th and June 7th, 1995,using the MPN (most probable number) method with two nutrient-poor media; a filter-sterilized lake water medium (LW-medium; ca. 1.7 mg C/l) and a diluted peptone medium (LT10^<-3>; ca. 2 mg C/1). The number of oligotrophs was generally 300-fold higher than the number of colony forming units on LT10^<-1> agar plates containing 0.5 g of peptone per liter (ca. 200 mg C/1), and corresponded to more than 25% of the total bacterial count determined by a direct counting method with DAPI staining. Most oligotrophs (97.5%; average) were obligate oligotrophs (OO), growing only at low nutrient concentrations with no growth on LT10^<-1> solid or liquid media. Five OO strains were isolated from LT10^<-3> and LW-medium in May, and approximately sequences of a region of 500 nucleotides of their 16S rRNA gene (16S rDNA) were determined. Phylogenetic analysis of 16S rDNA showed that the OO isolates were clearly distinguishable from the six strains of platable bacteria, which were isolated from the LT10^<-1> agar plate medium at the same time. Moreover, all platable bacteria were closely related to Comamonas testosteroni (β-proteobacteria subgroup), while all OO isolates with the exception of LO-52 were phylogenetically distant from every wellknown bacteria in the database and were widely distributed into proteobacterial subgroups. These results strongly suggest that there are many unknown OO living in freshwater environments, and that OO isolates will provide important information regarding microbial ecology.

• 日本微生物生態学会の論文
• 1997-06-30

著者

• Ishida Y

  Fukuyama Univ. Hiroshima Jpn

• Ishida Yuzaburo

  The Center For Environmental Research Ritsumeikan University

• UCHIDA Aritsune

  Laboratory of Marine Microbiology, Department of Applied Bioscience, Graduate School of Agriculture,

• Shin Mi-sun

  Laboratory Of Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Kyoto Uni

• Yoshinaga Ikuo

  Laboratory Of Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Kyoto Uni

• Yoshinaga Ikuo

  Laboratory Of Marine Microbiology Department Of Applied Biosciences Graduate School Of Agriculture K

• Uchida A

  Graduate School Of Agriculture Kyoto University

• Uchida Aritsune

  Laboratory Of Marine Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Ky

• KATANOZAKA NORIAKI

  Laboratory of Microbiology, Department of Applied Bioscience, Graduate School of Agriculture, Kyoto

• Katanozaka Noriaki

  Laboratory Of Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Kyoto Uni

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