Fine adhesion patterning and aligned nuclei orientation of mesenchymal stem cell on narrow line-width of silicone rubber implanted by carbon negative ions (第50回真空に関する連合講演会プロシーディングス)

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Fine cell-adhesion patterning and nuclei orientation control on silicone rubber were investigated by limiting line-width of carbon negative-ion implantation. The ions were implanted at 3×1015 ions/cm2 and 10 keV through masks of ridge-pattern and rectangle-pattern with various slit apertures of 0-40 μm in width. After 2 days culture, nuclei of rat mesenchymal stem cells were stained with fluorescent dye to evaluate the nuclei positions at each tip ridge-region and the relative angles between the major axis of ellipse-shape nuclei and the implanted line-region. Results showed the individual nucleus arrangement along the narrow line-widths of 3-12 μm and the gathered one on the wider line-width. Number of nuclei orientation in the angle range of 0°-10° increased to 79% on the line-width of 10 μm. As a result, the fine cell-adhesion patterning and nuclei orientation could be achieved by decreasing the implanted line-width to 10 μm.
2010-03-20