Participation of ATP in Nonadrenergic, Noncholinergic Relaxation of Longitudinal Muscle of Wistar Rat Jejunum
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概要
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A role of ATP in nonadrenergic, noncholinergic (NANC) relaxations was examined in the Wistar rat jejunum. Electrical field stimulation (EFS) induced NANC relaxation of longitudinal muscle of the jejunal segments in a frequency-dependent manner. A purinoceptor antagonist, adenosine 3-phosphate 5-phosphosulfate (A3P5PS, 100 μM) inhibited the relaxation: relaxations induced by EFS at lower or higher frequencies were either completely or partially inhibited, respectively. After the jejunal segments had been desensitized to ATP, the relaxations were decreased to the same extent as those inhibited by A3P5PS. An inhibitor of small conductance Ca2+-activated K+ channels (SK channels), apamin (100 nM), completely inhibited EFS-induced relaxations. Treatment of the segments with an inhibitor of sarcoplasmic reticulum Ca2+-ATPase, thapsigargin (1 μM), significantly inhibited the relaxations. The exogenous ATP-induced relaxation of longitudinal muscle occurred with a concomitant decrease in intracellular Ca2+ levels. Apamin and thapsigargin abolished these ATP-induced responses. A3P5PS significantly inhibited the inhibitory junction potentials which were induced in the longitudinal muscle cells. In addition, apamin significantly inhibited the hyperpolarization that was induced by exogenous ATP in the cells. These findings in the Wistar rat jejunum suggest that ATP participates in the NANC relaxation via activation of SK channels induced by Ca2+ ions that are released from the thapsigargin-sensitive store site.
- 社団法人 日本薬理学会の論文
- 2005-01-20
著者
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FUJITA Akikazu
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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TAKEUCHI Tadayoshi
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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HATA Fumiaki
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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Hata Fumiaki
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Hata Fumiaki
大阪府立大学 農学生命科学研究科
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OKISHIO Yutaka
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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TAKEWAKI Tadashi
Department of Veterinary Physiology, Gifu University
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WASEDA Kaori
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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OHTA Mayuko
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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Ohta Mayuko
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Waseda Kaori
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Okishio Y
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Fujita A
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Fujita Akikazu
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Takeuchi Tadayoshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Okishio Yutaka
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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TAKEWAKI Tadashi
Department of Pathogenetic Veterinary Science, United Graduate School, Gifu University
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HATA Fumiaki
Department of Pharmacology I, Osaka University School of Medicine
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