Localization of Ca^<2+> -Activated K^+ Channel, SK3, in Fibroblast-Like Cells Forming Gap Junctions With Smooth Muscle Cells in the Mouse Small Intestine
スポンサーリンク
概要
- 論文の詳細を見る
In the present study, we examined the expression and the localization of apamin-sensitive small conductance Ca2+-activated K+ channels (SK channels) in the mouse intestine. SK3-immunoreactivity (IR) was detected in both ileum and colon. Double staining experiments showed that SK3-IR was colocalized with prolyl 4-hydroxylase (PH(α))-IR, but not with c-Kit-IR which are markers of fibroblast cells and the interstitial cells of Cajal (ICC), respectively. Although SK3-IR was colocalized with vimentin-IR, which is another marker of ICC, the reactivity of SK3-immunopositive cells was weaker than that of ICC. The SK3-immunopositive cells were similarly present in the intestine of c-Kit mutant mice (W/WV), in which ICC were absent, and its wild-type mice. The immuno-electron microscopic analysis indicated that SK3 was localized in the cells that had some similar morphological features to ICC, but obviously different from ICC. The SK3-immunopositive cells had gap junctions with the smooth muscle cells. The gap junctions were smaller than those between ICC and smooth muscle cells. These results indicate expression of SK3 in fibroblast-like cells, but not in ICC, and suggest participation of the cells in the intestinal motility.
- 社団法人 日本薬理学会の論文
- 2003-05-01
著者
-
FUJITA Akikazu
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
-
TAKEUCHI Tadayoshi
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
-
HATA Fumiaki
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
-
Hata Fumiaki
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
-
Hata Fumiaki
大阪府立大学 農学生命科学研究科
-
JUN Hanai
Department of Pathology and Research, Sakai Municipal Hospital
-
Jun Hanai
Department Of Pathology And Research Sakai Municipal Hospital
-
Fujita A
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
-
Fujita Akikazu
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
-
Takeuchi Tadayoshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
-
HATA Fumiaki
Department of Pharmacology I, Osaka University School of Medicine
関連論文
- PAC1 Receptor-Mediated Relaxation of Longitudianal Muscle of the Mouse Proximal Colon
- Ascending contraction and descending relaxation in the distal colon of mice lacking interstitial cells of Cajal
- Examination of the role of cholinergic myenteric neurons with the impairment of neural reflexes in the ileum of c-kit mutant mice
- Participation of ATP in Nonadrenergic, Noncholinergic Relaxation of Longitudinal Muscle of Wistar Rat Jejunum
- Role of the Interstitial Cells Distributed in the Myenteric Plexus in Neural Reflexes in the Mouse Ileum
- Essential Role of the Interstitial Cells of Cajal in Nitric Oxide-Mediated Relaxation of Longitudinal Muscle of the Mouse Ileum
- Department of Ca^-Induced Contraction on ATP in α-Toxin-Permeabilized Preparations of Rat Femoral Artery
- Essential Role of ATP Synthesized by Creatine Kinase in Contraction of α-Toxin Permeabilized Preparations of Tonic Type Smooth Muscle
- Localization of Ca^ -Activated K^+ Channel, SK3, in Fibroblast-Like Cells Forming Gap Junctions With Smooth Muscle Cells in the Mouse Small Intestine
- The Site Where Newly Synthesized ATP Is Necessary for Tension Development in α-Toxin Permeabilized Preparations of Rat Proximal Colon
- Origin of Ca^ for Carbachol-Induced Contraction in Longitudinal Muscle of the Proximal Colon of Rats
- Effect of 1DMe, a Neuropeptide FF Analog, on Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- Mechanism of a Nitric Oxide Donor NOR 1-Induced Relaxation in Longitudinal Muscle of Rat Proximal Colon
- Mediators and Intracellular Mechanisms of NANC Relaxation of Smooth Muscle in the Gastrointestinal Tract
- Possible Role of Potassium Channels in Mu-receptor-Mediated Inhibition and Muscarinic Autoinhibition in Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) Inhibits Cyclic GMP-PKG Pathway-Independent Nonadrenergic, Noncholinergic Relaxation in Longitudinal Muscle of the Rectum of Wistar-ST Rats
- Mediators of Nonadrenergic, Noncholinergic Relaxation in Longitudinal Muscle of the Intestine of ICR Mice
- Decrease in Participation of Nitric Oxide in Nonadrenergic, Noncholinergic Relaxation of Rat Intestine With Age
- Inhibitory Effect of Endomorphin-1 and -2 on Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- Relationship Between Inhibitory Effect of Endogenous Opioid via Mu-Receptors and Muscarinic Autoinhibition in Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- Muscarinic Autoinhibition and Modulatory Role of Protein Kinase C in Acetylcholine Release from the Myenteric Plexus of Guinea Pig Ileum
- Nonadrenergic, Noncholinergic Relaxation in Longitudinal Muscle of Rat Jejunum
- Cooperation of ATP and Norepinephrine in Inducing Contraction in Guinea Pig Vas Deferens Is Not Associated with Change in Intracellular Ca^Level
- Characterization of antimuscarinic effect of cimetropium bromide in guinea pig ileum
- Relationship Between Muscarinic Autoinhibition and the Inhibitory Effect of Morphine on Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- Essential Role of Newly Synthesized ATP for Cyclic GMP-Induced Relaxation in α-Toxin Permeabilized Smooth Muscle of Rat Proximal Colon
- Effect of 1DMe, a Neuropeptide FF Analog, on Acetylcholine Release From Myenteric Plexus of Guinea Pig Ileum
- The Site Where Newly Synthesized ATP Is Necessary for Tension Development in α-Toxin Permeabilized Preparations of Rat Proximal Colon
- PAC1 Receptor-Mediated Relaxation of Longitudianal Muscle of the Mouse Proximal Colon
- 細胞膜糖脂質のナノスケール分布解析
- IS056 Identification of Critical Amino Acid Residues in the Extracellular Linker Region Responsible for the Difference in the Unitary Conductance of SUR/Kir6.0 Complex Channels
- PI-05 Quantitative electron microscopy shows uniform incorporation of triglycerides into existing lipid droplets
- WS5-02 Lipid droplet : ER fusion structure in hepatocytes(Histochemistry of Protein Function)
- 細胞膜糖脂質のナノスケール分布解析
- CONFORMATIONAL CHANGES IN ZYMOGEN GRANULES FROM THE PAROTID GLANDS INDUCED BY ATP AND A LOW CONCENTRATION OF CALCIUM
- CHARACTERISTICS OF [3H]E-643-BINDING TO ALPHA ADRENOCEPTORS
- DIRECT INHIBITORY EFFECT OF HEMICHOLINIUM-No. 3 ON ACETYLCHOLINE RELEASE
- SUBCELLULAR DISTRIBUTION OF ASCORBIC ACID IN RAT BRAIN
- EFFECTS OF SYNAPTIC PLASMA MEMBRANES ON RELEASE OF ACETYLCHOLINE FROM SYNAPTIC VESICLES
- INCREASE IN ACh RELEASE FROM THE RAT BRAIN CORTEX SLICES BY 3, 5-CYCLIC ADENOSINE MONOPHOSPHATE
- ROLE OF α-ADRENERGIC RECEPTORS IN DENERVATION SUPERSENSITIVITY OF RAT VAS DEFERENS
- CHANGES IN α-ADRENERGIC RECEPTORS IN RAT BRAIN IN VITRO BY PREINCUBATION WITH α-ADRENERGIC LIGANDS
- EFFECTS OF Na+ AND OTHER MONOVALENT CATIONS ON Ca-EFFLUX FROM SYNAPTOSOMES
- Role of PGE2 in Neurotransmission from Pre- to Post-Ganglionic Hypogastric Nerves of Guinea Pigs.
- Cooperation of ATP and norepinephrine in inducing contractile responses in guinea pig vas deferens.
- Inhibitory effect of endogenous macromolecule on the Ca++-stimulated acetylcholine release from the crude synaptic vesicles.
- Supersensitivity of amylase secretion from rat parotid tissue - its selective nature for the .BETA.2-adrenergic response.
- Dual effects of clonidine on amylase secretory responses of rat parotid tissue induced by .BETA.- and .ALPHA.1-agonists.
- Role of Cyclic AMP in Prostaglandin-Induced Modulation of Acetylcholine Release from the Myenteric Plexus of Guinea Pig Ileum.
- Contribution of external Ca2+ to the modulation by prostaglandin E2 of the release of acetylcholine from the myenteric plexus of guinea pig ileum.
- Effects of H-89, an inhibitor of protein kinase A, on the acetylcholine release from myenteric plexus of guinea pig ileum.
- Forskolin induces supersensitivity of the amylase secretory response of rat parotid tissue.
- Modulation of acetylcholine release from guinea-pig ileum myenteric plexus by arachidonic acid cascade inhibitors.