Mechanism of a Nitric Oxide Donor NOR 1-Induced Relaxation in Longitudinal Muscle of Rat Proximal Colon
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概要
- 論文の詳細を見る
We previously suggested that nitric oxide (NO)-mediated relaxation of the rat proximal colon is not associated with change in cyclic GMP content. We further studied the intracellular mechanism of NO-induced relaxation by measuring changes in tension and intracellular Ca2+ concentration ([Ca2+]i), simultaneously. NOR 1, NO donor, relaxed the longitudinal muscle of the rat proximal colon, which was precontracted by carbachol, with a concomitant decrease in [Ca2+]i. ODQ, an inhibitor of soluble guanylate cyclase, partially inhibited the relaxant effect of only higher concentrations of NOR 1, but Rp-8-Br-cGMPS, an inhibitor of cyclic GMP-dependent protein kinase (PKG), did not have any effects on the relaxant effect of NOR 1. When the preparations were transferred to normal solution after the treatment with thapsigargin, an inhibitor of sarcoplasmic reticulum (SR) Ca2+-ATPase, in the absence of Ca2+, contraction with a concomitant increase in [Ca2+]i occurred. NOR 1 did not show significant effects on the tension and [Ca2+]i in thapsigargin-treated preparations. In high K+-precontracted preparations, NOR 1 relaxed the preparations with a slight change in [Ca2+]i. The relaxant effect was significantly inhibited by ODQ and Rp-8-Br-cGMPS. These results suggest that NO induces the relaxation preferentially by acting thapsigargin-sensitive function of SR and in turn decreasing [Ca2+]i, although a cyclic GMP-PKG pathway is suggested under the experimental conditions of a high K+ concentration.
- 社団法人 日本薬理学会の論文
- 2001-08-01
著者
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FUJITA Akikazu
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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TAKEUCHI Tadayoshi
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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HATA Fumiaki
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, Osaka Prefe
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Hata Fumiaki
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Hata Fumiaki
大阪府立大学 農学生命科学研究科
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SUGIMOTO Keisuke
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science
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MORIMOTO Hajime
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Science
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Fujita A
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Fujita Akikazu
Department Of Anatomy And Molecular Cell Biology Nagoya University Graduate School Of Medicine
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Takeuchi Tadayoshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences Osaka Prefect
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Morimoto Hajime
Department Of Cardiovascular Medicine Shinshu University Graduate School Of Medicine
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Morimoto Hajime
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Science
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Sugimoto Keisuke
Department Of Pediatrics Kinki University School Of Medicine
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Sugimoto Keisuke
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Science
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HATA Fumiaki
Department of Pharmacology I, Osaka University School of Medicine
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