Simultaneous LC-MS/MS Analysis of the Plasma Concentrations of a Cocktail of 5 Cytochrome P450 Substrate Drugs and Their Metabolites
スポンサーリンク
概要
- 論文の詳細を見る
A "cocktail" approach, which involves simultaneous administration of multiple CYP-specific probes, concurrently detects the activity of multiple CYP enzymes. We developed and validated a rapid and selective LC-MS/MS method for determining the plasma concentrations of 5 CYP probe drugs and metabolites (caffeine/paraxanthine, CYP1A2 substrate; losartan/losartan carboxylic acid (E3174), CYP2C9 substrate; omeprazole/5-hydroxyomeprazole, CYP2C19 substrate; dextromethorphan/dextrorphan, CYP2D6 substrate; and midazolam/1′-hydroxymidazolam, CYP3A4 substrate) by single-step extraction, followed by a single LC-MS/MS run. An Ostro™ 96-well plate was used for extraction of CYP substrates and metabolites from human plasma and urine. Following optimization of the chromatographic conditions, all the peaks were well separated, and retention times ranged between 4.4 and 11.7 min. The total run time for a single injection was within 13 min. The accuracy and precision values suggested that the assay had high accuracy and reliability in plasma and urine samples. No significant matrix interference was observed. To demonstrate the efficacy of this method, plasma and urine concentrations of 5 CYP probe substrates and their metabolites were determined after simultaneous oral administration of 5 drugs to 4 healthy volunteers. All the substrates and metabolites were detected over an 8 h period, and the plasma concentrations of each substrate at 8 h after administration were above the lower limit of quantification. Urine concentrations of drugs and their metabolic ratio were evaluated after the administration. In conclusion, the advantage of our cocktail approach is that it enables in vivo assessment of the activity of various drug-metabolizing enzymes in a single assay.
著者
-
Namiki Noriyuki
Department Of Earth And Planetary Sciences Kyushu University
-
Inui Naoki
Department Of Clinical Pharmacology & Therapeutics Hamamatsu University School Of Medicine
-
Uchida Shinya
Department Of Anesthesiology Gunma University Hospital
-
Takeuchi Kazuhiko
Department Of Clinical Pharmacology & Therapeutics
-
Watanabe Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
-
Namiki Noriyuki
Department of Pharmacy Practice & Science, School of Pharmaceutical Sciences, University of Shizuoka
-
Tanaka Shimako
Department of Pharmacy Practice & Science, School of Pharmaceutical Sciences, University of Shizuoka
-
Namiki Noriyuki
Department of Clinical Pharmacology & Therapeutics, Hamamatsu University School of Medicine
関連論文
- Novel Preparation of Intercellular Lipid Models of the Stratum Corneum Containing Stereoactive Ceramide
- Endovascular Stent-Grafting for Recurrent Aneurysm in Behcets Disease
- Heterotopic sebaceous glands in the esophagus : Histopathological and immunohistochemical study of a resected esophagus
- Solitary esophageal metastasis of breast cancer with 15 years' latency : A case report and review of the literature
- PE-150 Comparison of Dilated Cardiomyopathy and Coronary Artery Disease in Effectiveness of Dl-sotalol on the Recurrence of Ventricular Tachyarrhythmia(Arrhythmia, therapy-10 (A) PE25,Poster Session (English),The 70th Anniversary Annual Scientific Meeting
- Efficacy of Bepridil for Antiarrhythmic Drug Refractory Ventricular Tachyarrhythmias in ICD Patients with Structural Heart Disease(Arrhythmia, Pharmacological Therapy 4 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Possible Prevalence and Transmission of Acute Respiratory Tract Infections Caused by Streptococcus pneumoniae and Haemophilus influenzae among the Internally Displaced Persons in Tsunami Disaster Evacuation Camps of Sri Lanka
- RNA Editing and Short Variant of Serotonin 2C Receptor mRNA in Neuronally Differentiated NG108-15 Cells
- Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents(Pharmacology)
- Chronic Treatment With Imipramine Inhibits Cell Growth and Enhances Serotonin 2C Receptor mRNA Expression in NG 108-15 Cells
- PJ-093 Extracellular but not Intracellular Acidosis Suppresses Endothelial Function by Inhibiting Intracellular Calcium Responses(Endothelium(03)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- IS121 Regulation of capacitative Ca^2+ influx in human monocytes/macrophages by myosin light-chain kinase
- Regulation of Bradykinin-Stimulated Cation Entry Into Endothelial Cells by tyrosine Kinase
- Structure-Activity Relationships of Receptor Binding of 1,4-Dihydropyridine Derivatives(Pharmacology)
- Excessive nitric oxide production and cardiac dysfunction in patients with chronic renal failure
- P-Glycoprotein Mediates Efflux Transport of Darunavir in Human Intestinal Caco-2 and ABCB1 Gene-Transfected Renal LLC-PK1 Cell Lines
- PJ-566 Influence of CYP2C9 Genotype on Pharmacokinetics and Pharmacodynamics of Benzbromarone(Cardiovascular pharmacology, basic/clinical-6, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-561 Antiproliferative Effect of Cyclic AMP on Human Umbilical Vein Endothelial Cells through TGF-beta Inducing p21(Cip1/Waf1) Expression(Cardiovascular pharmacology, basic/clinical-6, The 71st Annual Scientific Meeting of the Japanese Circulation Socie
- PJ-337 The significance of Delayed Enhancement MRI to Evaluate Global and Local Left Ventricular Wall Function in Hypertrophic Cardiomyopathy(Cardiomyopathy, basic/clinical-5 (M) PJ57,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meetin
- OJ-002 Influence of Genetic Polymorphisms of OATP-C on Cholesterol Lowering Effect of Pravastatin(Cardiovascular pharmacology, basic/clinical-2 (H) OJ1,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation
- 4 Inhibition of Type-5 Phosphodiesterase : Promising Therapy for Pulmonary Hypertension(Which drug is useful for the treatment of pulmonary hypertension?,Topic 4 (TP4) (M),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Long-Term Survival in Patients with Pulmonary Hypertension Treated with a Type 5 Phosphodiesterase Inhibitor Sildenafil(Pulmonary Circulation 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- OE-271 Pharmacokinetic and pharmacodynamic interactions between simvastatin and diltiazem in patients with hypercholesterolaemia and hypertension(Cardiovascular Pharmacology, Basic/Clinical 1 (H) : OE34)(Oral Presentation (English))
- Assessment of Forearm Reactive Hyperemia Using Near-Infrared Time-Resolved Spectroscopy : A New Tool to Evaluate Endothelial Function
- A Case of Addison's Disease Confirmed with Low Dose Cosyntropin Stimulation Test
- Association of Chlamydia Pneumoniae Infection and Carotid Intima-Media Wall Thickness in Japanese Americans
- PE-386 Clinical Impact after Cessation of Antiplatelet Therapy in Patients with Drug-eluting Stent Implantation(Cardiovascular pharmacology, basic/clinical(04)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Socie
- PE-085 Free Fatty Acids (FFAs), Arachidonic and Linoleic Acids but not other FFAs, Suppress Endothelial Function by Inhibiting Endothelial Ca^ Responses(Endothelium(02)(IHD),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Ci
- OE-243 Regenerated Endothelium Produces not Vasodilative but Vasoconstrictive Prostanoid(s) via Cyclooxygenase-dependent Pathway(Chronic coronary heart disease/Remodeling(01)(IHD),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japane
- PE-384 Direct Effect of ACE Inhibitors on Endothelium : ACE Inhibitors Enhance Kallikrein-induced Ca2+ Responses via Endogenous Bradykinin in Engothelial Cells(Cardiovascular pharmacology, basic/clinical-3, The 71st Annual Scientific Meeting of the Japane
- Large Quantities of NO Inhibit Agonist-Induced Endothelial Cell Ca^ Responses via a Peroxynitrite Pathway
- Inhibition of Type 5 Phosphodiesterase : Promising Therapy for Pulmonary Hypertension
- A potential role of sildenafil in the management of pulmonary hypertension
- A novel nonpeptide bradykinin receptor agonist in the regulation of endothelial calcium signaling and coronary artery tone
- Nitric oxide : a negative feedback on endothelium-derived vascular relaxing factors
- Inhibitory Effects of Corymine-Related Compounds on Glycine Receptors Expressed in Xenopus Oocytes
- Fermi-Surface Reconstruction in the Periodic Anderson Model(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Effect of the Insulin-like growth factor I receptor (IGF-IR) on radiosensitivity of human glioblastoma cells grown in multicellular spheroids.
- Quantifying the resilience of plant communities under different grazing intensities in a degraded shrubland : A case study in Mandalgobi, Mongolia
- Interaction between Amlodipine and Simvastatin in Patients with Hypercholesterolemia and Hypertension
- Antinociceptive Effects of St. John's Wort, Harpagophytum Procumbens Extract and Grape Seed Proanthocyanidins Extract in Mice(Pharmacology)
- Why Does Hemocyte Density Rise during the Wandering Stage of the Silkworm, Bombyx mori?
- Regeneration of Hemopoietic Organs in the Silkworm, Bombyx mori, After Locally Targeted Irradiation with Heavy Ion Beams
- Clinical Evaluation of Breech Deliveries Over a Fifteen-Year Period at a Hospital in Ota, Japan
- Autoimmune Hepatitis Overlapping with Primary Sclerosing Cholangitis
- Practical Assay Method of Cytosolic Acetoacetyl-CoA Thiolase by Rapid Release of Cytosolic Enzymes from Cultured Lymphocytes Using Digitonin
- An α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Antagonist and an N-Methyl-D-aspartate (NMDA) Channel Blocker Synergistically Impair Spatial Memory in Rats
- Magnetic Resonance Imaging Findings in Primary Amyloidosis-associated Arthropathy
- Expression Changes of the mRNA of Alzheimer's Disease Related Factors in the Permanent Ischemic Rat Brain(Pharmacology)
- DNA array analysis of gene expression changes by Choto-san in the ischemic rat brain
- Evaluation of the Nitric Oxide Radical Scavenging Activity of Manganese Complexes of Curcumin and Its Derivative(Pharmacology)
- Cytoprotective and Cytotoxic Effects of Curcumin : Dual Action on H_2O_2-Induced Oxidative Cell Damage in NG108-15 Cells
- Ameliorative Effects of Paeoniflorin, a Major Constituent of Peony Root, on Adenosine A_1 Receptor-Mediated Impairment of Passive Avoidance Performance and Long-Term Potentiation in the Hippocampus
- Paeoniflorin, a Major Constituent of Peony Root, Reverses Muscarinic M_1-Receptor Antagonist-Induced Suppression of Long-Term Potentiation in the Rat Hippocampal Slice
- Diazepam binding inhibitor (DBI) gene expression in the brains of socially isolated and group-housed mice
- GTS-21, a Nicotinic Agonist, Attenuates Multiple Infarctions and Cognitive Deficit Caused by Permanent Occlusion of Bilateral Common Carotid Arteries in Rats
- Chronic Cerebral Hypoperfusion-Induced Neuropathological Changes in Rats
- Low Dosage of Streptozotocin and Fat-diet Reduced Rat Liver Insulin Receptor Activities
- Evaluation on Rat Model of Metabolic Syndrome Induced by Fat-diet and Streptozotocin
- Tacrine Improves Working Memory Deficit Caused by Permanent Occlusion of Bilateral Common Carotid Arteries in Rats
- Effect of Pilocarpine on Striatal Acetylcholine Release in Dopamine-Depleted Rats
- Uptake of Liposomally Entrapped Fluorescent Antisense Oligonucleotides in NG108-15 Cells : Conventional versus pH-Sensitive
- Effect of Japanese Angelica Root Extract on Pentobarbital-Induced Sleep in Group-Housed and Socially Isolated Mice:Evidence for the Central Action
- Influence of Chronic Treatment With Imipramine on mRNA Levels in Rat Brain : Elevation of Glyceraldehyde-3-phosphate Dehydrogenase Levels
- Enhancement of Serotonin 2C Receptor mRNA Expression by Antidepressants Possessing the Receptor-Blocking Activity in the Rat Brain
- Molecular Cloning and Characterization of a Novel Sequence, vof-16, with Enhanced Expression in Permanent Ischemic Rat Brain(Pharmacology)
- In Vitro Antioxidant Activity of Vietnamese Ginseng Saponin and Its Components
- Studies on Development of Dosage Forms for Pediatric Use (V) Oral Mucosal Irritation Study of Gummi Drugs in Hamster Cheek Pouch
- Studies on Development of Dosage Form for Pediatric Use (No.4) Time Courses of Plasma Concentration of Acetaminophen after Oral Administration of Gummi Drug and Powder Preparation in Dogs
- Inhibition by Bacterial Lipopolysaccharide of Spontaneous and TNF-α-Induced Human Neutrophil Apoptosis In Vitro
- Auxiliary Heterotopic Partial Liver Transplantation in Pigs with Acute Liver Failure
- PJ-034 Omega-6 Polyunsaturated Fatty Acids, Arachidonic and Linoleic Acids, but not Other Free Fatty Acids, Suppress Endothelial Calcium Signaling and Functions(PJ006,Lipid Disorders (H),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The
- Blood-Brain Permeability of [^3H]-(3-methyl-His^2) Thyrotropin-releasing Hormone (MeTRH) in Mice : Effects of TRH and its Analogues
- In Vivo Measurement of 1, 4-Dihydropyridine Receptors in Mesenteric Arteries of Spontaneously Hypertensive Rats and Effect of Nifedipine and Cilnidipine
- Onset of Clinical Effects and Plasma Concentration of Fluvoxamine in Japanese Patients
- Effects of Cigarette Smoking and Cytochrome P450 2D6 Genotype on Fluvoxamine Concentration in Plasma of Japanese Patients
- I-123 MIBG Scintigraphy for the Evaluation of Cardiac Sympathetic Nerve Activity (SNA) : Comparison with Impulse Response Function (IRF)
- Sildenafil, a Type-5 Phosphodiesterase Inhibitor, Improves Vascular Response to Reactive Hyperemia in Patients with Raynaud's Phenomenon
- PE-187 The Interaction of NO and 20-HETE in the Reduction of Cerebral Blood Flow Following Subarachnoid Hemorrhage in Rats(Cerebrovascular circulation/Stroke-2 (IHD) PE32,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japan
- PE-073 Novel Property of Amlodipine to Improve Disturbed Endothelial Barrier Function by Mechanisms Other than the Effect on Voltage-dependent Calcium Channel(Cardiovascular pharmacology, basic/clinical-3 (H) PE13,Poster Session (English),The 70th Anniver
- The Role of TGF-alpha for Eosinophilic Sinusitis
- Is SPECT Cost-Effective in Managing Ischemic Heart Disease (IHD) in Japan? Comparison with Exercise ECG-Testing (EET) and Coronary Angiography (CAG)
- Metaiodobenzylguanidine and Heart Rate Variability in Heart Failure
- Remodeling in Chronic Sinusitis and Nasal Polyps
- Benzbromarone Pharmacokinetics and Pharmacodynamics in Different Cytochrome P450 2C9 Genotypes
- Formulation Study for Orally Disintegrating Tablet Using Partly Pregelatinized Starch Binder
- Testing hypotheses for the origin of steep slope of lunar size-frequency distribution for small craters
- Adequacy of control of preoperative hypertension can affect landiolol-induced hemodynamic changes in elderly patients during emergence from anesthesia
- PJ-173 Five-Year Survival in Patients with Pulmonary Arterial Hypertension Treated with the Combination of Sildenafil and Beraprost(Pulmonary circulation(03)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Societ
- Correlation between Peripheral Blood T-cell Profiles and Clinical and Inflammatory Parameters in Stable COPD
- Pulmonary Arterial Hypertension Associated With Connective Tissue Disease and Immunosuppressive Therapy
- Taste-Masking Effect of Physical and Organoleptic Methods on Peppermint-Scented Orally Disintegrating Tablet of Famotidine Based on Suspension Spray-Coating Method
- Linoleic Acid Attenuates Endothelium-Derived Relaxing Factor Production by Suppressing cAMP-Hydrolyzing Phosphodiesterase Activity
- Clinical Disintegration Time of Orally Disintegrating Tablets Clinically Available in Japan in Healthy Volunteers
- Nondestructive Evaluation of Setting and Hardening of Cement Paste Based on Ultrasonic Propagation Characteristics
- Plasma Concentration and Occupation of Calcium (Ca2+) Antagonist Receptors of Amlodipine, a Long-acting Ca2+ Antagonist in SHR
- Simultaneous LC-MS/MS Analysis of the Plasma Concentrations of a Cocktail of 5 Cytochrome P450 Substrate Drugs and Their Metabolites
- Incremental Shuttle Walk Test as a Valuable Assessment of Exercise Performance in Patients With Pulmonary Arterial Hypertension
- Comparison of Inhibitory Duration of Grapefruit Juice on Organic Anion-Transporting Polypeptide and Cytochrome P450 3A4
- The relation of gallstone and gastric mucosal lesion incidences in mice under different dietary conditions and possible mechanism.