ProgesteroneによるhCG生成, 分泌の選択的抑制と特異的hCG (α, β) mRNAの推移:in vitro絨毛培養系での検討

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In order to elucidate the inhibitory regulation of hCG synthesis, the dynamics of the synthesis and secretion of hCG was investigated by culturing explants of trophoblastic tissues from early placenta and choriocarcinoma in the presence and absence of progesterone, and these results were then compared to those of hPL. The dynamics of mRNAs encoding hCG (α, β) and hPL was assessed by grain counts in the tissue sections hybridized in situ with labelled cDNA probes corresponding to these mRNAs. In the control culture of early placenta, hCG, hCGαa and hPL in the medium showed marked increases compared to the initial tissue levels, whereas hCG, hCGα and immunoreactive hCGβ in the tissue explants remained constant. Progesterone suppressed the secretion of hCG and hCGα by placental tissue after 48-hour culture in a dose-response manner and simultaneously decreased the tissue level of immunoreactive hCGβ. However, neither hPL secretion nor hPL tissue level were decreased by progesterone. The <I>in situ</I> hybridization revealed that grain counts for hCGα-mRNA and hCGβ-mRNA in the sections prepared from placenta cultured with progesterone were decreased compared to those prepared without progesterone, where-as grain counts for hPL-mRNA remained unchanged. In culture of explants from chorio-carcinoma, no inhibition of synthesis and secretion of hCG by progesterone was found. From these results it will be concluded that progesterone inhibits hCG synthesis through the inhibition of hCGα-mRNA and hCGβ-mRNA accumulation and that regulation of hCG synthesis in choriocarcinoma is different from that in the normal placenta.
一般社団法人日本内分泌学会の論文

ProgesteroneによるhCG生成, 分泌の選択的抑制と特異的hCG (α, β) mRNAの推移:in vitro絨毛培養系での検討

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