ISOLATION AND IDENTIFICATION OF GREEN FLUO-RESCENT COMPOUND ACCUMULATED IN NON-GROWING CELLS OF EREMOTHECIUM ASHBYII BY THE ADDITION OF GLYOXAL

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The addition of a trapping agent, glyoxal, to a non-growing cell medium with Eremothecium ashbyii brought about the accumulation of large quantities of a green fluorescent compound, with a simultaneous rigid inhibition of riboflavin formation. The fluorescent compound was isolated from the mycelia after non-growing cell incubation and highly purified to a crystalline form through various column chromatographic steps. The purified compound was identified as 8-ribityllumazine by comparison with a synthesized reference compound by means of spectro photometric and fluorometric measurements. Furthermore, it was verified that glyoxal, at the added concentration, and 8-ribityllumazine, at the accumulated concentration, caused slight inhibition of riboflavin formation with riboflavin synthetase from E, ashbyii. Accordingly, it was concluded that the 8-ribityllumazine accumulated is a derivative compound of an intermediate in flavinogenesis which is 4-ribitylamino 5-amino-2, 6-dihydroxypyrimidine based on the trapping action of glyoxal.
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