Biosynthesis of Thiamine in Plants:I. Enzymic Formation of Thiamine from Pyrimidine and Thiazole Moieties
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概要
- 論文の詳細を見る
The enzyme preparations obtained from acetone powders of 5 kinds of green leaves contained the enzyme system responsible for the synthesis of thiamine from pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine) and thiazole (4-methyl-5-β-hydroxyethylthiazole) moieties. The condensation reaction of the moieties was dependent upon the presence of ATP and Mg2+. The enzyme preparations showed the optimal activity at around pH 7.5 and lost its activity abruptly beyond 48°.The rate of thiamine synthesis increased with the increase of ATP concentration and reached an optimum. Beyond the optimum, the rate decreased as ATP concentration increased. The additional Mg2+ not only accelerated the rate but also partially removed the inhibition by ATP. In addition to Mg2+, Mn2+ and Co2+ also activated the thiamine synthesis.
- 日本ビタミン学会の論文
著者
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田中 孝男
Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto University
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満田 久輝
Laboratory of Nutritional Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyoto Univeristy
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河合 文雄
Laboratory of Food Science and Technology, Research Institute for Production Development
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