FORMATION OF GUANINE RIBONUCLEOTIDYL-(3-5)-ADENOSINE IN A FLAVINOGENIC STRAIN OF EREMOTHECIUM ASHBYII

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概要

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• The addition of caffeine caused the accumulation of a new nucleotide compound simultaneously with the rigid inhibition of ribo flavin production in non-growing cells of Eremothecium ashbyii. In the present study we tried to identify the structure of the nucleotide compound using non-growing cells of the mold. 1) It became possible to obtain a large amount of mycelia by massculti vation in a reagent tank. 2) A new nucleotide compound, referred to as compound A in the paper, was extracted with perchloric acid solution and purified by the following subsequent procedures: 1) Dowex 1×2 (HCOO-) column, 2) charcoal treatment, 3) DEAE-Sephadex A25 (Cl-) column, 4) Dowex 1×2 (Cl-) column, and 5) DEAE-Sephadex A25 (HCO3-) column. 3) The structure of the new nucleotide compound was proved to be guanine ribonucleotidyl-(3-5)-adenosine (GpA) from the results of the following analyses: 1) alkaline degradation, 2) UV-spectra, IR-spectra and NMR-spectra, and 3) enzymatic treatments with RNase T2 and phosphodiesterase. 4) The roles of caffeine and guanine ribonucleotidyl-(3-5)-adenosine in connection with flavinogenesis of this mold were discussed.

• 財団法人 学会誌刊行センターの論文

著者

• 西川 善之

  京大農栄養化学

• 満田 久輝

  Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto University

• 中島 謙二

  Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto University

• 西川 善之

  Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto University

• 中島 謙二

  Laboratory of Nutritional Chemistry, Faculty of Agriculture, Kyoto Univeristy

• 満田 久輝

  Laboratory of Nutritional Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

• 満田 久輝

  Laboratory of Nutritional Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyoto Univeristy

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