LC-MS/MSを用いた茶中の残留農薬一斉分析法 : 厚生労働省通知一斉試験法の改良

概要

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An LC-MS/MS method for the simultaneous determination of pesticide residues in tea was developed by modifying the Japanese official multiresidue method. In the optimal sample preparation procedure, the following sequence of steps was adopted: (1) swelling of the sample in water; (2) extraction with acetonitrile; (3) removal of water by salting-out; (4) cleanup on an ODS column and then on a tandem graphitized carbon/PSA column. The resulting test solution was subjected to LC-MS/MS and determined by external solvent standard calibration. The recoveries for 135 pesticides from fortified green tea, black tea, oolong tea, and matcha (powdered green tea) after spiking at the Japanese maximum residue limits were mostly within the range 70-120%, with relative standard deviations of <20%. The test solutions obtained by the modified method were cleaner than those obtained by the original multiresidue method and contained relatively smaller amounts of pigments and other matrix components. No interfering peak was observed in the blank chromatograms, indicating the high selectivity of the modified method. Therefore, the developed method is considered to be highly efficient and suitable for the quantitative analysis of pesticide residues in tea.
2014-04-25