定量NMRを用いたダッタンソバ乾麺中のクエルセチンの迅速定量

概要

論文の詳細を見る
Quantitative NMR (qNMR) method was applied for the quantification of quercetin in tartary buckwheat (Fagopyrum tataricum L.) noodle. In the reagent market, quercetin is generally provided as quercetin +X hydrate of which the purity is not determined exactly. Hence, if using the reagent as the reference material for LC quantification, the reliability of analysis data will not be assured. qNMR is based on the fact that the signal intensities of a given NMR resonance are directly proportional to the molar amount of that nucleus in the sample, and is able to determine the contents with traceability to International System of Units (SI units). The content of quercetin was calculated from the ratio of the signal intensities of a proton at H-2' on quercetin to eighteen protons of the methyl groups on hexamethyldisilane (HMD) used as the internal standard, after the concentration of HMD was corrected using potassium hydrogen phthalate (PHP), which is one of certified reference material (CRM). In the result, the content of quercetin in tartary buckwheat noodle was determined with SI-traceability to 1.58±0.14mg/g as the anhydrate formula. The quantitative value was verified using general LC method after the purity of reagent was determined exactly. qNMR does not need its reference compound, the calibration curve and separation column like LC method. Our procedure in this study is rapid and simple with overall analysis time of only 10min, and also the result is traceable to SI units.
2010-12-20

定量NMRを用いたダッタンソバ乾麺中のクエルセチンの迅速定量

スポンサーリンク

概要

著者

関連論文

スポンサーリンク