アセトニトリル不足に対応した食品中のサイクラミン酸分析法の改良

概要

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Corresponding to the world wide shortage of acetonitrile. HPLC conditions employed in the official method for determination of cyclamate in foods were improved because of it in the mobile phase. As the results, satisfactory separation of cyclamate and same detection limit of analyte as that of original HPLC conditions were obtained by using a methanol-water (80:20) as a mobile phase. To evaluate the applicability of improved HPLC conditions to actual food sample, 7 kinds of commercial foods were fortified, cleaned-up by official method and applied to HPLC analysis. Satisfactory chromatograms without any interfering peak and sufficient recoveries were obtained from every sample, suggesting that present HPLC conditions were applicable to the official method as those for separation and detection of cyclamate.
2010-04-14