SDS-PAGEによる既存添加物酵素のタンパク質分離パターン

概要

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Food manufacturing enzymes are named according to enzyme functions in Japan. Active enzyme proteins are not identified as substances. Therefore, in the List of Existing Food Additives, most items consist of enzymes from different origins. If a chemical analysis of proteins in enzyme products can identify origins, the method will be a probable candidate for a simple identification test for enzyme origins. SDS-PAGE was applied to α-amylases, β-amylases, catalases, β-galactosidases, glucoamylases, cellulases, proteases, and hemicellulases. As many products as possible were collected to cover most of currently circulating products in Japan. One hundred and three products available in Japan were analyzed by SDS-PAGE. Molecular weights of major proteins were calculated. Characteristic electrophoresis patterns were found in most products. Useful information on relationships between origins and separation patterns of proteins was obtained. Identification of species was achieved for all products from plants and many products from bacteria or fungi although identification was limited to the genera level for some microorganisms like Bacillus.
2010-08-23