陽イオン交換 : 逆相固相抽出カートリッジを用いたHPLCによる食品中のアスパルテーム分析法

概要

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A simple and rapid method for determination of aspartame(APM) in foods using Cation-Exchange and Reversed-Phase Cartridge, and high performance liquid chromatography(HPLC) was developed. APM in a sample was extracted with 0.01 mol/L hydrochloric acid in ultrasonic bath, and the extract was loaded onto an Oasis MCX, cation-exchange and reversed-phase cartridge. The cartridge was washed with water followed by methanol - acetonitrile (1:1), and APM was eluted with 5 % sodium chloride - methanol - acetonitrile (3:1:6). The eluate was evaporated to remove organic solvents, and the residue was made up to a volume of 10 mL with water. APM in this solution was chromatographed on a Inertsil ODS-SP (4.6 mm i.d. × 150 mm) column with 0.02 mol/L phosphate buffer, pH 5.0, - methanol (3:1) as the mobile phase and detected with an ultraviolet detector at 210 nm. The recoveries of APM from 12 kinds of food added at the level of 0.02 g/kg and 0.10 g/kg were 90〜100 %. The detection limit was 0.01 g/kg. We examined 13 kinds of commercial food samples labeled to contain APM by the proposed method. The quantitative values of APM in tested samples were 0.07〜12 g/kg.
2008-04-30