Polymorphism of Acid and Neutral α-Glucosidases in Banana Pulp : Changes in Apparent pIs and Affinity to Con A of the Enzymes during Ripening(Food & Nutrition)
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概要
- 論文の詳細を見る
We observed the presence of acid and neutral α-glucosidases with optimum pHs of 4.5 and 6.5, respectively, and their multiple forms in banana pulp. By chromatofocusing analysis, eachα-glucosidase from preclimacteric bananas was found to be of pI > 7.4, which disappeared upon ripening, although another form with pI 5 as a major component appeared instead. It is unknown whether this phenomenon is due to protein de novo synthesis and/or post-translational modifications of the enzyme. A large amount of acidα-glucosidase from preclimacteric bananas was shown to be a glycoprotein that bound to a Con A-Sepharose column. While this type of enzyme decreased during ripening, it remained a possibility that sugar components of the enzyme might be processed during ripening. The neutralα-glucosidase had no affinity to the column through ripening. These results provide preliminary information on the mechanism of appearance ofα-glucosidase multiforms as well as on a useful strategy for purification of acid and neutralα-glucosidases from banana pulp at various stages of ripening.
- 社団法人日本農芸化学会の論文
- 1991-04-23
著者
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Konishi Yuko
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Konishi Y
Kirin Brewery Co. Ltd. Gunma Jpn
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Konishi Yotaro
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
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Nakatani N
Osaka City Univ. Osaka Jpn
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Nakatani Nobuji
Department Of Food And Nutrition Faculty Of Human Life Science Osaka City University
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KITAZATO Sachiko
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University
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ASANO Ryoko
Department of Food and Nutrition, Fuculty of Science of Living, Osaka City University
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Asano Ryoko
Department Of Food And Nutrition Fuculty Of Science Of Living Osaka City University
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Kitazato Sachiko
Department Of Food And Nutrition Faculty Of Science Of Living Osaka City University:(present Address
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Konishi Yotaro
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University
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